Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/33)

Name: Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-116
Price: 329.0 USD
Availability: InStock

8 Citations

Our Anti-mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N75/33. It detects human, mouse, and rat mGluR1/5 (Group I) glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].

Adult rat hippocampus immunohistochemistry.

Electron micrograph shows N75/33 immunolabelling in the stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunogold particles were observed at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts (Den) of pyramidal cells establishing synaptic contacts with excitatory axon terminals (b). 80.000 X. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).

Adult rat brain membrane immunoblot.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

Trial Size

SKU: 75-116

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mGluR1/5 (Group I) glutamate receptor

Scientific Background

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N75/33

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Grm5 Gprc1e Mglur5

Molecular Weight

130 kDa (both mGluR1 and mGluR5)

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession number P31424) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2232873

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.44

Conjugate

Unconjugated

Specificity

Recognizes mGluR1 and mGluR5

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Metabotropic glutamate receptor 5 (mGluR5)

UniProt ID

P31424

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

Immunocytochemistry: Mouse
Western Blot: Human | Mouse | Rat
Additional Publications: Unspecified

Immunocytochemistry: Mouse
PMID	Dilution	Publication
30926797	1:200	Edfawy, M., et al. 2019. Abnormal mGluR-mediated synaptic plasticity and autism-like behaviours in Gprasp2 mutant mice. Nature Communications, 1431.

Western Blot: Human
PMID	Dilution	Publication
31658977	1:1000	Granatiero, V., et al. 2019. Nrf2 signaling links ER oxidative protein folding and calcium homeostasis in health and disease. . Life science alliance, e201900563.
29752072	not listed	Endo, R., et al. 2018. TDP-43 and DISC1 Co-Aggregation Disrupts Dendritic Local Translation and Mental Function in FTLD. Biological Psychiatry, 509-521.
24332449	1:500	Pretto, D.I., et al. 2014. Reduced excitatory amino acid transporter 1 and metabotropic glutamate receptor 5 expression in the cerebellum of fragile X mental retardation gene 1 premutation carriers with fragile X-associated tremor/ataxia syndrome.. Neurobiology of Aging, 1189-1197.

Western Blot: Mouse
PMID	Dilution	Publication
29752072	not listed	Endo, R., et al. 2018. TDP-43 and DISC1 Co-Aggregation Disrupts Dendritic Local Translation and Mental Function in FTLD. Biological Psychiatry, 509-521.
24069373	not listed	Breiderhoff, T., et al. 2013. Sortilin-related receptor SORCS3 is a postsynaptic modulator of synaptic depression and fear extinction.. PLoS One, e75006.

Western Blot: Rat
PMID	Dilution	Publication
24103031	1:800	Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons.. FEBS J, 6114-27.

Additional Publications: Unspecified
PMID	Publication
31256144	Post, J.I., et al. 2019. Differential Levels and Phosphorylation of Type 1 Inositol 1,4,5-Trisphosphate Receptor in Four Different Murine Models of Huntington Disease. Journal of Huntingtons Disease, 271-289.
25627107	Valero, M.L., et al. 2015. TRPC1 and metabotropic glutamate receptor expression in rat auditory midbrain neurons.. Journal of Neuroscience Research, 964-972.

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Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/33)

SKU: 75-116

Product Details

Product Specific References for Applications and Species

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