Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/3)

Name: Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-115
Price: 329.0 USD
Availability: InStock

7 Citations

Our Anti-mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N75/3. It detects human, mouse, and rat mGluR1/5 (Group I) glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Adult rat hippocampus immunohistochemistry.

Adult rat brain membrane immunoblot.

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

SKU: 75-115

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mGluR1/5 (Group I) glutamate receptor

Scientific Background

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N75/3

Isotype

IgG2a

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Grm5 Gprc1e Mglur5

Molecular Weight

130 kDa (both mGluR1 and mGluR5)

Immunogen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession number P31424) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_10672260

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.43

Conjugate

Unconjugated

Specificity

Recognizes mGluR1 and mGluR5

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Metabotropic glutamate receptor 5 (mGluR5)

UniProt ID

P31424

Shipping

Shipped on ice packs

Product Specific References for Applications and Species

ELISA: Mouse
Western Blot: Mouse | Rat
Additional Publications: Unspecified

ELISA: Mouse
PMID	Dilution	Publication
34852231	not listed	Lautz, J.D., et al. 2021. Synaptic protein interaction networks encode experience by assuming stimulus-specific and brain-region-specific states. Cell Reports, 110076.
30237867	not listed	Brown, E.A., et al. 2018. Clustering the autisms using glutamate synapse protein interaction networks from cortical and hippocampal tissue of seven mouse models. Molecular Autism, 48.

Western Blot: Mouse
PMID	Dilution	Publication
23553633	1:2000	Cao, Z., et al. 2013. Enhanced asynchronous Ca2+ oscillations associated with impaired glutamate transport in cortical astrocytes expressing Fmr1 gene premutation expansion. . Journal of Biological Chemistry, 13831-13841.
23112146	not listed	Harraz, M.M., et al. 2012. MicroRNA-223 is neuroprotective by targeting glutamate receptors.. PNAS USA, 18962-18967.

Western Blot: Rat
PMID	Dilution	Publication
24103031	1:800	Luo, P., et al. 2013. Downregulation of postsynaptic density-95-interacting regulator of spine morphogenesis reduces glutamate-induced excitotoxicity by differentially regulating glutamate receptors in rat cortical neurons. FEBS Journal, 6114-6127.

Additional Publications: Unspecified
PMID	Publication
26306919	Lv, J.M., et al. 2015. The Noncompetitive AMPAR Antagonist Perampanel Abrogates Brain Endothelial Cell Permeability in Response to Ischemia: Involvement of Claudin-5. Cellular and Molecular Neurobiology, 745-753.
19224364	Smalla, K.H., et al. 2009. Altered postsynaptic-density-levels of caldendrin in the para-chloroamphetamine-induced serotonin syndrome but not in the rat ketamine model of psychosis.. Neurochemical Research, 1405-1409.

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Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/3)

SKU: 75-115

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody

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