Anti-Metabotropic Glutamate Receptor 5/1a Antibody

Name: Anti-Metabotropic Glutamate Receptor 5/1a Antibody
Brand: PhosphoSolutions
SKU: 2032-mGluR5/1
Price: 456.0 USD
Availability: InStock

Our Anti-Metabotropic Glutamate Receptor 5/1a primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat Metabotropic Glutamate Receptor 5/1a and is antigen affinity purified. It is great for use in WB, IHC.

Western blot of 10 µg of HEK 293 cells expressing: 1) mGluR5 and 2) mGluR1a showing the specific immunolabeling of the ~125 kDa monomer and the ~250 kDa dimers of both mGluR1a (2) and mGluR5 (1).

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Species Reactivity

Mouse, Rat

Applications

ELISA, IHC, WB

Host Species

Rabbit

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Data Sheet

Trial Size

SKU: 2032-mGluR5/1

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Metabotropic Glutamate Receptor 5/1a

Scientific Background

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

ELISA, IHC, WB

Host Species

Rabbit

Gene Name

GRM5

Molecular Weight

125/250 kDa

Immunogen

Synthetic peptide corresponding to amino acid residues from the C-terminal region of rat mGluR5 and rat mGluR1a, conjugated to keyhole limpet hemocyanin (KLH).

Immunogen Species

Rat

Species Reactivity

Mouse, Rat

Expected Reactivity

Human

Antibody Registry ID

AB_2492152

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Prepared from pooled rabbit serum using a column to which the peptide immunogen was coupled.

Buffer

10 mM HEPES (pH 7.5), 150 mM NaCl, 100 µg per ml BSA and 50% glycerol.

Dilution Ranges

WB: 1:1000
IHC: 1:500

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~125 kDa monomer and the ~250 kDa dimers of mGluR5 and mGluR1. Immunolabeling blocked by preadsorption of antibody with the peptide used to generate the antibody.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

glutamate receptor metabotropic 5, GPRC1EMGLUR5metabotropic glutamate receptor 5, mGlu5, mGluR5

UniProt ID

P31424

NCBI Gene ID

24418

Shipping

Blue Ice

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Anti-Metabotropic Glutamate Receptor 5/1a Antibody

SKU: 2032-mGluR5/1

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