Anti-MECP2 Antibody (N227/21)

Name: Anti-MECP2 Antibody
Brand: NeuroMab
SKU: 75-273
Price: 337.0 USD
Availability: InStock

Our mouse MECP2 monoclonal antibody from NeuroMab is produced in-house from clone N227/21. It detects human, mouse, and rat MECP2 and is purified by Protein A chromatography. Works in IHC, ELISA.

Adult rat hippocampus immunohistochemistry.

Serial dilutions of BSA-conjugated modified and unmodified peptides dotted onto membrane and probed with N227/21 (left) and loading control (right) TC supe.

Adult rat hippocampus immunohistochemistry.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, IHC

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-273

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

MECP2

Scientific Background

MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair and is not influenced by sequences flanking the methyl-CpGs. MeCP2 has been shown to mediate transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A (Nan et al., 1998). Defects in MeCP2 are the cause of Rett syndrome (RTT) (Amir et al., 1999). RTT is an X-linked dominant disease; it is a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N227/21

Isotype

IgG1

Applications

ELISA, IHC

Host Species

Mouse

Gene Name

MECP2

Molecular Weight

75 kDa

Immunogen

Synthetic peptide amino acids 157-169 (FTVTG[dimethyl-R]GSPSRRE) of human MECP2 (accession number P51608)

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_11001583

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Reacts preferentially with dimethylated peptide than with unmethylated peptide

Quality Control

Each new lot of antibody is quality control tested by IHC on either rat or mouse brain and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Methyl-CpG-binding protein 2 (MeCp-2 protein) (MeCp2)

UniProt ID

P51608

Shipping

Shipped on ice packs

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Anti-MECP2 Antibody (N227/21)

SKU: 75-273

Product Details

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