Anti-mDia2 (C-terminal region) Antibody

Name: Anti-mDia2 (C-terminal region) Antibody
Brand: PhosphoSolutions
SKU: DP3491
Price: 340.0 USD
Availability: InStock

7 Citations

Our mDia2 (C-terminal region) rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat mDia2 (C-terminal region) and is antigen affinity purified. It is great for use in WB.

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

Immunofluorescent images showing Parvalbumin (hair cells, red) and Diaph3 (Cat. DP3491, 1:400, magenta) expression in cryo-sectioned cochlea (organ of Corti area). The knockdown of the endogenous Stub1 in mouse inner ear

Immunofluorescent images showing Parvalbumin (SGNs, red) and Diaph3 (Cat. DP3491, 1:400, magenta) expressions in cryo-sectioned cochlea (SGN area).The knockdown of the endogenous Stub1 in mouse inner ear leads to severe

Western blot analysis of mDia2 expression in rat PC12 (lane 1), human A431 (lane 2), mouse brain (lane 3), and rabbit spleen fibroblasts (lane 4). The blots were probed with anti-mDia2 (C-terminal region) at 1:500.

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

Host Species

Rabbit

Go to Full Product Details

Data Sheet

SKU: DP3491

Volume: 100 µL

Variant

Price:

$340.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mDia2 (C-terminal region)

Scientific Background

Formins include several families of proteins that regulate actin cytoskeletal dynamics via two conserved formin homology domains, FH1 and FH2. The FH1 region contains poly-proline stretches that promote interactions with profilin. The FH2 domain, located C-terminally to the FH1 domain, is highly conserved in formin proteins and possesses actin nucleation and polymerization activities. Through cooperation of FH1 and FH2, formins construct actin-based structures comprising linear, unbranched filaments that are used in stress fibers, actin cables, microspikes, and contractile rings. A subgroup of the formins is the diaphanous (Dia) family, which includes mDia1 (Diap1), mDia2 (Diap3), and mDia3 (Diap2). The mDia2 protein has been implicated in cell migration and cytokinesis. This Dia protein can nucleate actin polymerization, as well as bind and stabilize microtubules. mDia2 may also have functions in the nucleus, since it is continually shuttled between the cytoplasm and nucleus

Format

Antigen Affinity Purified

Clonality

Polyclonal

Isotype

IgG

Applications

Host Species

Rabbit

Gene Name

DIAPH2

Molecular Weight

135

Immunogen

mDia2 synthetic peptide (coupled to KLH) corresponding to amino acid residues in the C-terminal region of mouse mDia2 (Diap3). This peptide sequence is highly conserved in rat and human mDia2, and has low homology to other formins.

Immunogen Species

Mouse

Species Reactivity

Human, Mouse, Rat

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Buffer

PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol

Dilution Ranges

WB: 1:1000

Conjugate

Unconjugated

Specificity

The antibody detects a 135 kDa* protein corresponding to the apparent molecular mass of mDia2 on SDS-PAGE immunoblots of various cells and tissues, including human A431 and HeLa cells, mouse brain tissue and C2C12 cells, rat PC12 cells, and rabbit spleen fibroblasts.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Country of Origin

United States

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

Diap3, Dia2, Drf3, formin

UniProt ID

Q9NSV4

Shipping

Blue Ice

Product Specific References for Applications and Species

Immunocytochemistry: Human | Rat
Immunohistochemistry: Mouse
Western Blot: Human | Mouse

Immunocytochemistry: Human
PMID	Dilution	Publication
26287480	not listed	Belin, BJ, et al. 2015. DNA damage induces nuclear actin filament assembly by Formin -2 and Spire-½ that promotes efficient DNA repair.. eLife, e07735.

Immunocytochemistry: Rat
PMID	Dilution	Publication
26912794	not listed	Monzo, P, et al. 2016. Mechanical confinement triggers glioma linear migration dependent on formin FHOD3. Molecular biology of the cell, 1246-1261.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
39322015	1:400	Qiu, C, et al. 2024. Stub1 promotes degradation of the activated Diaph3: A negative feedback regulatory mechanism of the actin nucleator. The Journal of Biological Chemistry, 107813.
34559979	not listed	Monzo, P, et al. 2021. Adaptive mechanoproperties mediated by the formin FMN1 characterize glioblastoma fitness for invasion. Developmental cell, 2841-2855.

Western Blot: Human
PMID	Dilution	Publication
29113998	not listed	Egami, Y, et al. 2017. RhoC regulates the actin remodeling required for phagosome formation during FcγR-mediated phagocytosis. Journal of cell science, 4168-4179.
23580645	not listed	Sandbo, N, et al. 2013. Control of myofibroblast differentiation by microtubule dynamics through a regulated localization of mDia2. The Journal of Biological Chemistry, 15466-15473.

Western Blot: Mouse
PMID	Dilution	Publication
34559979	not listed	Monzo, P, et al. 2021. Adaptive mechanoproperties mediated by the formin FMN1 characterize glioblastoma fitness for invasion. Developmental cell, 2841-2855.

Recover password

Create my account

Anti-mDia2 (C-terminal region) Antibody

SKU: DP3491

Product Details

Product Specific References for Applications and Species

Bulk Order Anti-mDia2 (C-terminal region) Antibody

Related Products

Recently Viewed