Anti-mCherry Antibody (1C51)

Name: Anti-mCherry Antibody
Brand: PhosphoSolutions
SKU: 1203-mCherry
Price: 456.0 USD
Availability: InStock

Our Anti-mCherry primary antibody from PhosphoSolutions is mouse monoclonal. It detects mCherry and tdTomato and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunostaining of HEK293 cells transfected with mCherry(red) and stained with anti-mCherry antibody (Cat. 1203-mCherry, green, 1:500) subsequently appear yellow. The nuclei stained blue (DAPI) distinguishes untransfected cells.

Western blot of mCherry transfected HEK293 cell lysate (+) showing specific immunolabeling of the ~28 kDa mCherry protein. Labeling is absent in the untransfected lysate (-).

Immunostaining of HEK293 cells transfected with mCherry(red) and stained with anti-mCherry antibody (Cat. 1203-mCherry, green, 1:500) subsequently appear yellow. The nuclei stained blue (DAPI) distinguishes untransfected cells.

Click on image to zoom

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1203-mCherry

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

mCherry

Scientific Background

mCherry is a red fluorescent protein derived from DsRed which is isolated from disc corals and related to GFP (green fluorescent protein) (Shaner et al., 2004). Fluorescent proteins are commonly used to tag proteins in the study of their expression and in transgenic research. Antibodies against fluorescent proteins such as mCherry are especially useful when amplification of the fluorescent signal is necessary or desired.

Format

Protein G Purified

Clonality

Monoclonal

Clone

1C51

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Molecular Weight

28 kDa

Immunogen

Recombinant full length protein

Antibody Registry ID

AB_2715492

Storage

Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

PBS + 50% glycerol and 5 mM NaN3

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Specific for the ~28 kDa mCherry protein

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

DSRED, red fluorescent protein mCherry, Red Fluoroscent Protein

Shipping

Blue Ice

Western Blot Protocol: Download

General References

Shaner, N. C., Campbell, R. E., Steinbach, P. A., Giepmans, B. N., Palmer, A. E., & Tsien, R. Y. (2004). Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nature Biotechnology, 22(12), 1567. PMID: 15558047

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Anti-mCherry Antibody (1C51)

SKU: 1203-mCherry

Product Details

General References

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