Anti-Mad3 (Human) Antibody (N129/15)

Name: Anti-Mad3 (Human) Antibody
Brand: NeuroMab
SKU: 75-250
Price: 337.0 USD
Availability: InStock

Our mouse Mad3 (human) monoclonal antibody from NeuroMab is produced in-house from clone N129/15. It detects human, Xenopus Mad3 (human) and is purified by Protein A chromatography. Works in WB, ICC, ELISA.

Transfected cell immunoblot: extracts of COS cells transiently transfected with HA-tagged mouse and human Mad3 (mMad3 and hMad3, respectively) or untagged Kv2.1 plasmid and probed with N129/15 (left) and K89/41 (right) TC supes.

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Species Reactivity

Human, Xenopus

Applications

ELISA, ICC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

SKU: 75-250

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Mad3 (human)

Scientific Background

MAX Dimerization Protein 3 is encoded by the gene MXD3. MXD3 is a member of the Myc superfamily. MXD3 is a transcriptional repressor which forms a heterodimer with the cofactor MAX to form a sequence-specific DNA-binding protein complex which recognizes the core sequence 5'-CAC[GA]TG-3'. MXD3 is expressed in lung and blood. Diseases associated with MXD3 include Perianal Hematoma and Bartholin's Duct Cyst.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N129/15

Isotype

IgG2b

Applications

ELISA, ICC, WB

Host Species

Mouse

Gene Name

MXD3 BHLHC13 MAD3

Molecular Weight

25 kDa

Immunogen

Fusion protein amino acids 116-206 (C-terminus) of human Mad3 (accession number Q9BW11) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Xenopus

Antibody Registry ID

AB_11001559

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

Does not cross-react with Mxi1

Quality Control

Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Max dimerization protein 3 (Max dimerizer 3) (Class C basic helix-loop-helix protein 13) (bHLHc13) (Max-associated protein 3) (Max-interacting transcriptional repressor MAD3) (Myx)

UniProt ID

Q9BW11

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Shipped on ice packs

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Anti-Mad3 (Human) Antibody (N129/15)

SKU: 75-250

Product Details

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