Anti-LRRK2/Dardarin, N-Terminus Antibody (8G10)

Name: Anti-LRRK2/Dardarin, N-Terminus Antibody
Brand: NeuroMab
SKU: 75-308
Price: 394.0 USD
Availability: InStock

Our mouse LRRK2/Dardarin, N-terminus monoclonal antibody from NeuroMab is produced in-house from clone 8G10. It detects human, mouse, and rat LRRK2/Dardarin, N-terminus and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Immunoblot versus crude membranes from adult rat brain (RBM) and wild-type (WT) and LRRK2 KO mouse brains probed with 8G10 (left), N241A/34 (middle) and K89/34 (right) TC supe. Mouse brain samples provided by Xiaojie Li, Ted Dawson and Valina Dawson, Johns Hopkins University

Adult rat brain immunohistochemistry (with antigen retrieval via sodium citrate pretreatment).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-308

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

LRRK2/Dardarin, N-terminus

Scientific Background

LRRK2 (also known as PARK8) encodes a protein with 5 putative functional domains: an N-terminal leucine-rich repeat (LRR) domain, a Roc (Ras of complex protein) domain that shares sequence homology to the Ras-related GTPase superfamily, a COR (C-terminal of Roc) domain, a mitogen-activated protein kinase kinase kinase (MAPKKK) domain, and a C-terminal WD40 repeat domain. Mutation in this gene is one of the most common causes of inherited Parkinson disease (Gandhi et al., 2008). LRRK2 was originally identified as a putative disease-causing transcript (DKFZp434H2111) within a 2.6-Mb region encompassing a locus for Parkinson disease-8 (PARK8). Northern blot analysis detected a 9-kb mRNA transcript in all tissues tested, including brain. The authors named the protein product dardarin, derived from the Basque word dardara, meaning tremor. LRRK2/dardarin is also known to positively regulate autophagy through a calcium-dependent activation of the CaMKK/AMPK signaling pathway and together with RAB29, plays a role in the retrograde trafficking pathway for recycling proteins, such as mannose 6 phosphate receptor (M6PR), between lysosomes and the Golgi apparatus in a retromer-dependent manner. LRRK2/PARK8 is also known to regulate neuronal process morphology in the intact central nervous system (CNS) and play a role in synaptic vesicle trafficking.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

8G10

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

LRRK2 PARK8

Molecular Weight

>200 kDa

Immunogen

Fusion protein amino acids 100-500 (N-terminus) of human LRRK2 (accession number Q5S007) produced recombinantly in E. Coli.

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2315882

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Conjugate

Unconjugated

Specificity

No cross-reactivity reported

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Leucine-rich repeat serine/threonine-protein kinase 2 (EC 2.7.11.1) (Dardarin)

UniProt ID

Q5S007

Shipping

Shipped on ice packs

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Anti-LRRK2/Dardarin, N-Terminus Antibody (8G10)

SKU: 75-308

Product Details

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