Anti-LAMP1 Antibody (5H6)

Name: Anti-LAMP1 Antibody
Brand: PhosphoSolutions
SKU: 1030-LAMP1
Price: 456.0 USD
Availability: InStock

Our Anti-LAMP1 primary antibody from PhosphoSolutions is mouse monoclonal. It detects human LAMP1 and is Protein G purified. It is great for use in WB, IHC, ICC.

Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (Cat. 1030-LAMP1, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (Cat. 2105-VIM, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.

Western blot of HeLa cell lysate showing specific immunolabeling of the ~100 kDa LAMP1 protein.

Immunostaining of HeLa cells showing specific, strong cytoplasmic labeling of LAMP1 (Cat. 1030-LAMP1, red, 1:2000) corresponding to lysosomes and late endosomes. Additional staining with Anti-Vimentin (Cat. 2105-VIM, green, 1:500) specifically labeling the intermediate filament network. The blue stain is DAPI to reveal nuclear DNA.

Immunolabeling of HeLa cells showing vesicular staining of LAMP1 protein (Cat. 1030-LAMP1, red, 1:2000) accumulated in swollen lysosomes, while Anti-Vimentin (Cat. 2105-VIM, green, 1:500) specifically labels the intermediate filament network in these cells. The blue stain is DAPI to reveal nuclear DNA.

Click on image to zoom

Species Reactivity

Human

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

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Data Sheet

Trial Size

SKU: 1030-LAMP1

Volume: 100 µL

Variant

Price:

$456.00

Quantity:

Ships: 1-2 business days

Product Details

Target

LAMP1

Scientific Background

Lysosomal Associated Membrane Protein1 (LAMP1) is a protein that is localized primarily in lysosomes but may also be present on late endosomes and the plasma membrane. LAMP1 antibodies are therefore widely used as lysosome markers. It has recently been suggested that lysosomes are activated in microglia in the progression of multiple system atrophy (MSA) and thus play a key role in its pathology (Makioka et al., 2012).

Format

Protein G Purified

Clonality

Monoclonal

Clone

5H6

Isotype

IgG1

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

LAMP1

Molecular Weight

100 kDa

Immunogen

Recombinant full length human LAMP1 expressed in and purified from E. coli.

Immunogen Species

Human

Species Reactivity

Human

Antibody Registry ID

AB_2492140

Storage

Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Physical State

Liquid

Production Notes

Protein G purified culture supernatant

Buffer

PBS + 10 mM NaN3.

Dilution Ranges

WB: 1:5000
IHC: 1:2000
ICC: 1:500-1:2000

Conjugate

Unconjugated

Specificity

Specific for endogenous levels of the ~100 kDa LAMP1 protein.

Quality Control

Western blots performed on each lot.

Usage Statement

For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.

Expiration

After date of receipt, stable for at least 1 year at -20°C.

Synonyms

CD107 antigen like family member A antibody, CD107 antigen-like family member A antibody, CD107a antibody, CD107a antigen antibody, LAMP 1 antibody, LAMP-1 antibody, LAMP1 antibody, LAMP1_HUMAN antibody, LAMPA antibody, LGP120 antibody, lgpA antibody, Lysosomal membrane glycoprotein 120KD antibody, Lysosomal Associated Membrane Protein 1 antibody, Lysosome associated membrane glycoprotein 1 antibody, Lysosome-associated membrane glycoprotein 1 antibody, Lysosome-associated membrane protein 1 antibody, OTTHUMP00000040663 antibody

UniProt ID

P11279

NCBI Gene ID

3916

Shipping

Blue Ice

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Anti-LAMP1 Antibody (5H6)

SKU: 1030-LAMP1

Product Details

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