Anti-Kv4.3 K+ Channel Antibody (K75/41)

Name: Anti-Kv4.3 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-017
Price: 394.0 USD
Availability: InStock

Our mouse Kv4.3 K+ channel monoclonal antibody from NeuroMab is produced in-house from clone K75/41. It detects drosophila, guinea pig, human, mouse, rabbit, rat, and Xenopus Kv4.3 K+ channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA.

Electron micrograph shows K75/41 immunolabelling in the hippocampus using a pre-embedding immunogold method. Immunogold particles (arrows) were observed along the extrasynaptic plasma membrane of dendritic shafts (Den) of interneurons establishing synaptic contacts with excitatory axon terminals (b). Scale bar = 500 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).

Adult rat hippocampus immunohistochemistry.

Immunoblot on adult rat brain membranes (RBM), and adult mouse brain membranes from wild-type (MBM-WT) and Kv4.3 knockout (MBM-Kv4.3-KO) mice. MBM samples courtesy of Dr. Jeanne Nerbonne, Washington University School of Medicine.

Immunoblots on brain membranes prepared from whole rat (RBM) and mouse (MBM) brain, and from human cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].

Electron micrograph shows K75/41 immunolabelling in the hippocampus using a pre-embedding immunogold method. Immunogold particles (arrows) were observed along the extrasynaptic plasma membrane of dendritic shafts (Den) of interneurons establishing synaptic contacts with excitatory axon terminals (b). Scale bar = 500 nm. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).

Click on image to zoom

Species Reactivity

Drosophila, Guinea Pig, Human, Mouse, Rabbit, Rat, Xenopus

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-017

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv4.3 K+ channel

Scientific Background

Kv4.3 K+ channel (Potassium voltage-gated channel subfamily D member 3 ) is a member of the mammalian Shal-related family and the potassium voltage gated channel subunit family. Kv4 subunit family includes four Kv4 α subunits (Kv4.1, Kv4.2 and Kv4.3). However only Kv4.2 and Kv4.3 are predominantely epressed in the brain (Alfaro-Ruiz et al., 2005). KV4.3 subunit is encoded by gene KCND3 in human. It has been demonstrated that Kv 4.2 and Kv4.3 are often colocalized with pain-modulating molecules in rat spinal lamina II excitatory interneurons (Huang et al., 2005).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K75/41

Isotype

IgG1

Applications

ELISA, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Kcnd3

Molecular Weight

75 kDa in rat brain membrane preparations

Immunogen

Fusion protein amino acids 415-636 (cytoplasmic C- terminus) of rat Kv4.3 (accession number Q62897) produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Drosophila, Guinea Pig, Human, Mouse, Rabbit, Rat, Xenopus

Antibody Registry ID

AB_2131966

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:500
ICC: 1:100

Conjugate

Unconjugated

Specificity

No cross-reactivity against rat Kv4.2 expressed in transfected cells.

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium voltage-gated channel subfamily D member 3 (Voltage-gated potassium channel subunit Kv4.3)

UniProt ID

Q62897

Shipping

Shipped on ice packs

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Anti-Kv4.3 K+ Channel Antibody (K75/41)

SKU: 75-017

Product Details

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