Anti-Kv4.2 K+ Channel Antibody (K57/1)

Name: Anti-Kv4.2 K+ Channel Antibody
Brand: NeuroMab
SKU: 75-016
Price: 394.0 USD
Availability: InStock

Our mouse Kv4.2 K+ channel monoclonal antibody from NeuroMab is produced in-house from clone K57/1. It detects C. elegans, human, mouse, and rat Kv4.2 K+ channel and is purified by Protein A chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA, EM.

Immunofluorescence staining of adult wild-type (WT) and Kv4.2 knockout (Kv4.2-/-) mouse hippocampus with K57/1 (red) and Kv2.1 rabbit (green).

Immunoblot against crude brain membranes from adult rat (RBM) and wild-type (MBM-WT) and Kv4.2 knockout (MBM-Kv4.2-KO) mice probed with K57/1 (left) or K89/41 (right) TC supe.

Confocal images of mouse cerebellar sagittal (left) or transverse (right) slices stained with a Kv4.2 antibody (Cat. 75-016; red). AAV-GABRα6-GFP (green) was injected on the indicated postnatal days. Image from publication CC-BY-4.0. PMID:37095324

Confocal images showing tdT- (red) and GFP-positive GC dendrites (green) included in glomeruli, which are visualized by staining with three antibodies, Kv4.2 (Cat. 75-016; purple in left panels or blue in right panels), vGLUT1 (blue), and vGAT (blue). Glomeruli are shown with arrows. Image from publication CC-BY-4.0. PMID:37095324

Immunoblot against crude C. elegans worm extracts and RBM.

Immunoblot against crude membranes from whole mouse (MBM) or rat (RBM) brain and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)] or hippocampus [HBM(H)] and probed with K57/1 (left) or N52A/42 loading control (right) TC supe.

Immunofluorescence staining of adult wild-type (WT) and Kv4.2 knockout (Kv4.2-/-) mouse hippocampus with K57/1 (red) and Kv2.1 rabbit (green).

Click on image to zoom

Species Reactivity

C. elegans, Human, Mouse, Rat

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-016

Volume: 100 µL

Variant

Price:

$394.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Kv4.2 K+ channel

Scientific Background

Kv4.2 K+ channel (Potassium voltage-gated channel subfamily D member 2 ) is a member of the mammalian Shal-related family and potassium voltage gated channel subunit family. Kv4 subunit family includes four Kv4 α subunits (Kv4.1, Kv4.2 and Kv4.3). However only Kv4.2 and Kv4.3 are predominantely expressed in the brain (Alfaro-Ruiz et al., 2019). Kv4.2 K+ is encoded by gene KCND2 in human. It helps to regulate the action potential's circadian rhythm firing in suprachiasmatic nucleus neurons. This process regulates the circadian rhythm of locomotor activity. It has been demonstrated that Kv 4.2 and Kv4.3 are often colocalized with pain-modulating molecules in rat spinal lamina II excitatory interneurons (Huang et al., 2005).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

K57/1

Isotype

IgG1

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

KCND2 KIAA1044

Molecular Weight

75-80 kDa

Immunogen

Synthetic peptide amino acids 209 –225 (extracellular S1-S2 loop) of human Kv4.2 (CGSSPGHIKELPSGERY; accession number Q63881)

Immunogen Species

Human

Species Reactivity

C. elegans, Human, Mouse, Rat

Antibody Registry ID

AB_2131945

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:250

Conjugate

Unconjugated

Specificity

No cross reactivity against rat Kv4.3

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Potassium voltage-gated channel subfamily D member 2 (Voltage-gated potassium channel subunit Kv4.2)

UniProt ID

Q9NZV8

Shipping

Shipped on ice packs

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Anti-Kv4.2 K+ Channel Antibody (K57/1)

SKU: 75-016

Product Details

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