Our mouse Kv2.1 K+ channel (external) monoclonal antibody from NeuroMab is produced in-house from clone K39/25. It detects human, mouse, rat, snail, and zebrafish Kv2.1 K+ channel (external) and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.
Surface immunofluorescence (no detergent permeabilization) of cultured rat hippocampal neuron transfected with HA-tagged Kv2.1 plasmid. Red = K39/25. Scale bar = 20 m. Reproduced from 2000 Lim et al Neuron (PMID 10719893).
Potassium voltage-gated channel subfamily B member 1 is encoded by the gene KCNB1. KCNB1 is a member of the potassium channel family, B (Shab) (TC 1.A.1.2) subfamily, Kv2.1/KCNB1 sub-subfamily. KCNB1 is a delayed rectifier potassium channel that mediates transmembrane potassium transport. KCNB1 contributes to the regulation of action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells, and endocrine cells, and plays a role in homeostatic attenuation of electrical excitability throughout the brain. KCNB1 is expressed in the brain, heart, lung, liver, colon, kidney, and adrenal gland. Diseases associated with KCNB1 include early infantile epileptic encephalopathy 26, undetermined early-onset epileptic encephalopathy, visual epilepsy, and alacrima, achalasia, and mental retardation syndrome.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
K39/25
IgG2a
ELISA, ICC, IHC, WB
Mouse
KCNB1
105-125 kDa (varies with cell background due to phosphorylation)
Synthetic peptide amino acids 211-229 (LPELQSLDEFGQSTDNPQL, extracellular S1-S2 loop) of rat Kv2.1 (accession number NP_037318)
Rat
Human, Mouse, Rat, Snail
AB_2131649
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.85
Unconjugated
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
United States
24 months from date of receipt
Potassium voltage-gated channel subfamily B member 1 (Delayed rectifier potassium channel 1) (DRK1) (h-DRK1) (Voltage-gated potassium channel subunit Kv2.1)
