Our Anti-Glycine Receptor rabbit polyclonal primary antibody from PhosphoSolutions is produced in-house. It detects human, mouse, and rat Glycine Receptor and is antigen affinity purified from pooled serum. It is great for use in WB, IHC.
Western blot of rat spinal cord lysate (SC) showing specific immunolabeling of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling was absent from a rat hippocampal lysate (H), as the glycine receptor is not expressed in the hippocampus.
Glycine is an important inhibitory transmitter in the brainstem and spinal cord. Glycine receptors are members of the ligand-gated ion channel family (LGICs) that mediate rapid chemical neurotransmission (Schofield et al., 2003). The binding of glycine to its receptor produces a large increase in chloride conductance, which causes membrane hyperpolarization. Glycine receptors are anchored at inhibitory chemical synapses by a cytoplasmic protein, gephyrin (Fischer et al., 2000). The glycine receptor has been used to great advantage in the identification of the binding sites for alcohol on the LGIC family of proteins (Beckstead et al., 2001; Mihic et al., 1997). These receptors have also been extremely useful in studies of synaptic clustering of receptors (Craig and Lichtman, 2001). The glycine receptor may also act in concert with an NMDAR subunit to form an excitatory receptor (Chatterton et al., 2002).
Antigen Affinity Purified from Pooled Serum
Polyclonal
IgG
ELISA, IHC, WB
Rabbit
GLRA1
48 kDa
Synthetic peptide corresponding to amino acid residues from the N-terminal region of the α1 subunit of rat glycine, conjugated to keyhole limpet hemocyanin (KLH).
Rat
Human, Mouse, Rat
AB_2492133
The antibody should be reconstituted in 50 µl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term.
Lyophilized
Prepared from pooled rabbit serum by affinity purification using a column to which the fusion protein immunogen was coupled.
Lyophilized from 5 mM ammonium biocarbonate.
WB: 1:1000
WB Brain: 1:1000
IHC: 1:1000
Unconjugated
Specific for endogenous levels of the ~48 kDa α1- and α2-subunits of the glycine receptor. Immunolabeling is blocked by preadsorption of the antibody with the peptide immunogen. Does not recognize other glycine receptor subunits.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
