Anti-Glutamic Acid Decarboxylase-67 (GAD 67) Antibody

Name: Anti-Glutamic Acid Decarboxylase-67 (GAD 67) Antibody
Brand: Aves Labs
SKU: GAD
Price: 481.0 USD
Availability: InStock

This affinity-purified antipeptide antibody was generated in chickens against a sequence shared between the mouse (CAA01912) and human (NP_000808) gene products.

Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).

Immunohistochemical staining of inhibitory neurons (red; 1:500) within the hippocampal formation of a transgenic, adult male Black6 mouse brain. The brains were fixed with 4.0% paraformaldehyde. The green staining is low level GFP fluorescence expressed off the actin promoter in this mouse transgenic strain. Secondary antibody (Texas Red goat anti-chicken IgY, (1:500).

Immunocytochemical staining of GAD-67 (red/yellow) in cultured cortical neurons from a neonatal mouse (C57/Black6). GAD staining was detected using Texas Red goat anti-chicken IgY (1:500). MAP2 staining (green) used a rabbit antibody (1:500). Hoda Ilias, Aves Labs.

Sph21 neuroblastoma cells in culture. Fixed with 4% paraformaldehyde. GAD.1 (green, 1:1000 dilution) immunoreactivity. Phalloidin (F-actin) (red) staining. DAPI (blue) staining. Photomicrograph from Page Balich (Univ. Arizona).

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Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, WB

Host Species

Chicken

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Data Sheet

Trial Size

SKU: GAD

Volume: 1000 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Glutamic Acid Decarboxylase-67 (GAD 67)

Scientific Background

Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter beta-amino butyric acid (GABA). It is also know as GAD-1.

Format

Affinity-Purified IgY

Concentration

100 µg/mL

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, IHC, WB

Host Species

Chicken

Gene Name

GAD1 GAD GAD67

Molecular Weight

67 kDa

Immunogen

Synthetic peptide

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2313545

Storage

Store at 4°C in the dark. Under these conditions, the antibodies should have a shelf life of at least twelve months, provided they remain sterile. For longer term storage, aliquot and freeze to avoid freeze-thaw of the antibody.

Physical State

Liquid

Production Notes

Chickens were immunized with a synthetic peptide / keyhole limpet hemocyanin (KLH) conjugate. This synthetic peptide corresponded to a region near the Cterminus of this gene product, and was 100% conserved between the human (Q99259, NCBI), mouse (P48318, NCBI) and rat (NP_058703, NCBI) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, the concentrations of the eluate adjusted to 100 µg/mL, and the preparation was filter-sterilized through a 0.45 µm filter.

Buffer

Phosphate-buffered (10 mM) isotonic (0.9%, w/v) saline (“PBS," pH 7.2) with sodium azide (0.02%, w/v) added as a preservative.

Dilution Ranges

WB: 1:1000-1:2000
IHC: 1:500-1:1000
ICC: 1:500-1:1000

Quality Control

This antibody preparation was analyzed by immunohisto-chemistry (at a concentration of 1 µg/mL) using fluorescein-labeled goat anti-chicken IgY (1:500 dilution, Aves Labs Cat.# F-1005) as the secondary reagent.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

12 months from receipt of product

Synonyms

Glutamate decarboxylase 1 (EC 4.1.1.15) (67 kDa glutamic acid decarboxylase) (GAD-67) (Glutamate decarboxylase 67 kDa isoform)

UniProt ID

Q99259

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Anti-Glutamic Acid Decarboxylase-67 (GAD 67) Antibody

SKU: GAD

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