Our mouse GluN2A/NR2A biotin conjugate monoclonal antibody from NeuroMab is produced in-house from clone N327/95. It detects human, mouse, rabbit, and rat GluN2A/NR2A and is purified by Protein A chromatography. Works in ELISA, IHC, ICC, WB.
Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.
Purified by Protein A chromatography
Lot dependent: provided at 0.3-0.5 mg/mL
Monoclonal
N327/95
IgG2a
ELISA, ICC, IHC, WB
Mouse
Grin2a
170 kDa
Fusion Protein Amino acids 75-325 (extracellular N-terminus) of rat NR2A (accession number Q00959) produced recombinantly in E. Coli
Rat
Human, Mouse, Rabbit, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography and conjugation of purified mAb. Purified mAbs are >90% specific antibody.
PBS with 0.09% azide
Biotin
Does not cross-react with GluN2B/NR2B
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
