Our mouse GluN2A/NR2A monoclonal antibody from NeuroMab is produced in-house from clone N327/95. It detects human, mouse, rabbit, and rat GluN2A/NR2A and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.
Glutamate receptor NMDA2A or GluN2A/NR2A glutamate receptor is a member of the glutamate-gated ion channel family. NR2A is a subunit of the ligand-gated ion channel with high calcium permeability and voltage-dependent sensitivity to magnesium. NR2A is expressed throughout the brain, including striatum, forbrain, cerebellum (at protein level) brain cortex, piriform cortex, hippocampus, caudate-putamen, dentate gyrus and granule cell layer. It is found at the postsynaptic cell membrane, dendritic spine, synapse, and cytoplasmic vesicle membrane. A variety of neurological diseases, including epilepsy, intellectual disability, autism spectrum disorders, developmental delay, and schizophrenia are associated with mutations in the NR2A gene.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
N327/95
IgG2a
ELISA, ICC, IHC, WB
Mouse
Grin2a
170 kDa
Fusion protein amino acids 75-325 (extracellular N-terminus) of rat NR2A (accession number Q00959) produced recombinantly in E. Coli
Rat
Human, Mouse, Rabbit, Rat
AB_2315842
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
WB: 1:500
IHC: 1:1000
ICC: 1:500
Unconjugated
Does not cross-react with GluN2B/NR2B
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
