Our mouse Glucagon monoclonal antibody from Antibodies Incorporated is produced in-house from clone 9. It detects human, pig, and rat Glucagon and is Protein A or Protein G purified. Works in ELISA, IF, IHC.
Glucagon is a peptide hormone of 29 amino-acid residues produced by the alpha cells of the pancreatic islets. Its effect is to raise blood sugar as a stress hormone and counter-regulatory hormone for insulin. It is produced by cell-type-specific processing of proglucagon, which in the alpha cells is split to form glicentin-related pancreatic polypeptide, glucagon and major proglucagon fragment, while the same precursor is split to form glicentin, oxyntomodulin (C-terminally extended glucagon-37), GLP-1 and GLP-2 in the L cells of the intestinal mucosa.
Protein A or Protein G purified
1 mg/mL ±15%
Monoclonal
9
IgG1/k
ELISA, IF, IHC
Mouse
GCG
20.4 kDa
Synthetic C-terminal fragment of glucagon coupled to carrier
Human
Human
2-8°C without exposure to light
Liquid
BSA free
0.01 M phosphate buffer, pH 7.4, with 0.5 M NaCl and 15 mM sodium azide
IHC: 1:8000
ABS 061-09 binds free glucagon in solution. ABS 061-09 when used as a capture antibody forms a sandwich ELISA pair with ABS 058-08 as a biotinylated detection antibody for measuring glucagon. ABS 061-09 was used in IHC in formaline fixed paraffin embedded tissue with a dilution of 1:8000. ABS 061-09 was used in IF with a dilution of 1:4000.
Unconjugated
ABS 061-09 binds human glucagon. Cross-reacts less than 5% with oxyntomodulin. The epitope lies within the C-terminal region of glucagon.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
