Anti-GluA2/GluR2 Glutamate Receptor Recombinant Chicken Chimeric mAb (L21/32)

Name: Anti-GluA2/GluR2 Glutamate Receptor Recombinant Chicken Chimeric mAb
Brand: Aves Labs
SKU: 78-002
Price: 481.0 USD
Availability: InStock

Our chicken Anti-GluA2/GluR2 glutamate receptor recombinant monoclonal antibody is a chimeric antibody derived from NeuroMab clone L21/32. It detects human, mouse, and rat GluA2/GluR2 glutamate receptor and is purified by affinity chromatography. KO validated. Works in WB, IHC, ICC, IP, ELISA, EM.

Representative section of formalin fixed, paraffin-embedded rat brain hippocampal region showing cells stained by anti-GluA2/GluR2 Glutamate Receptor recombinant chicken mAb (green, Cat No. 78-002) using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488).

$Western blotting of rat brain membrane with anti-GluA2/GluR2 Glutamate Receptor recombinant chicken mAb (Cat No. 78-002). The chicken mAb was detected with anti-chicken-HRP (Cat No. 80-1000-HRP) and recognizes endogenous GluA2 in the rat brain membrane fraction at ~100 kDa.$

Representative section of formalin fixed, paraffin-embedded rat brain hippocampal region showing cells stained by anti-GluA2/GluR2 Glutamate Receptor recombinant chicken mAb (green, Cat No. 78-002) using goat anti-chicken IgY CF®488 secondary antibody (Cat No. 80-1001-488).

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Species Reactivity

Human, Mouse, Rat

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Chicken

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

Trial Size

SKU: 78-002

Volume: 100 µL

Variant

Price:

$481.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GluA2/GluR2 glutamate receptor

Scientific Background

Glutelin type-A 2 (GluA2) , also called GRIA2, GLUR2, GLURB, GluA2, GluR-K2, HBGR2 or glutamate ionotropic receptor AMPA type subunit 2, is a member of the Glutamate receptor family of the mammalian brain. This neurotransmiter receptor subunit, which is activated during normal central nervous system fuction, is encoded by the GRIA2 (or GLUR2) gene. GluA2 constitutes a key subunit that regulates AMPA receptors. AMPA receptors lacking of Glua2 have been shown to be present in diseased brains, whose basic fuctions have been altered.

Format

Purified by affinity chromatography

Concentration

1 mg/mL

Clonality

Recombinant

Clone

L21/32

Isotype

IgY

Applications

ELISA, EM, ICC, IHC, IP, WB

Host Species

Chicken

Gene Name

Gria2 Glur2

Molecular Weight

90 kDa

Excitation / Emission

Unconjugated

Immunogen

Fusion protein amino acids 834-883 (EFCYKSRAEAKRMKVAKNPQNINPSSSQNSQNFATYKEGYNVYGIESVKI, cytoplasmic C-terminus) of rat GluA2/GluR2 produced recombinantly in E. Coli

Immunogen Species

Rat

Species Reactivity

Human, Mouse, Rat

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

This recombinant antibody is a chimeric antibody created by replacing the mouse heavy and light constant regions of clone L21/32 with chicken IgY heavy and light constant regions. As such this antibody retains the same binding performance as the original clone L21/32 but can be detected using standard anti-chicken secondary antibodies allowing flexibility for multiplexing applications. This antibody is expressed recombinantly in mammalian cells and then affinity purified from the cell culture media.

Buffer

1X PBS, 0.05% Sodium Azide pH 7.4

Dilution Ranges

WB: 1:500-1:1000
WB Brain: 1:500-1:1000
IHC: 1:250-1:500
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with GluA1/GluR1, GluA3/GluR3 or GluA4/GluR4 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by Western blot on rat brain membrane fraction and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

Glutamate receptor 2 (GluR-2) (AMPA-selective glutamate receptor 2) (GluR-B) (GluR-K2) (Glutamate receptor ionotropic, AMPA 2) (GluA2)

UniProt ID

P19491

Shipping

Shipped on ice packs

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Anti-GluA2/GluR2 Glutamate Receptor Recombinant Chicken Chimeric mAb (L21/32)

SKU: 78-002

Product Details

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