Anti-Glial Fibrillary Acidic Protein (GFAP) Antibody
Our Anti-Glial Fibrillary Acidic Protein (GFAP) primary antibody from PhosphoSolutions is chicken polyclonal. It detects bovine, human, mouse, and rat Glial Fibrillary Acidic Protein (GFAP) and is total IgY fraction. It is great for use in WB, IHC, ICC.
Immunofluorescence of a section of mouse hippocampus colabeled with Anti-GFAP (Cat. 621-GFAP, green, 1:5000) and Anti-FOX3 (red). The Anti-FOX3 labels the nuclei and proximal perikarya of neurons while the Anti-GFAP labels a network of astroglial cells. The blue is DAPI staining of nuclear DNA.
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SKU: 621-GFAP
Ships: 1-2 business days
Product Details
Glial Fibrillary Acidic Protein (GFAP)
Glial Fibrillary Acidic Protein (GFAP) was discovered by Amico Bignami and co-workers as a major fibrous protein of multiple sclerosis plaques (1). It was subsequently found to be a member of the 10nm or intermediate filament (IF) family, specifically the IF family Class III, which also includes peripherin, desmin and vimentin. GFAP is strongly and specifically expressed in astrocytes and certain other astroglia in the CNS, in satellite cells, peripheral ganglia, and in non-myelinating Schwann cells in peripheral nerves. In many damage and disease states GFAP expression is heavily upregulated in astrocytes. In addition, neural stem cells frequently strongly express GFAP. Point mutations in the protein coding region of the GFAP gene lead to Alexander disease which is characterized by the presence of abnormal astrocytes containing GFAP protein aggregates known as Rosenthal fibers (2).
Total IgY fraction
Polyclonal
IgY
ELISA, ICC, IHC, WB
Chicken
GFAP
50 kDa
Human recombinant protein
Bovine
Rat
AB_2492125
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Total IgY fraction
PBS + 10 mM NaN3.
WB: 1:10,000
WB Brain: 1:10,000
IHC: 1:1000-1:5000
ICC: 1:1000-1:5000
WB Brain: 1:10,000
IHC: 1:1000-1:5000
ICC: 1:1000-1:5000
Unconjugated
Specific for endogenous levels of the ~50 kDa GFAP protein. A lower band at ~45 kDa is a proteolytic fragment derived from the GFAP molecule.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
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281189
Blue Ice
- Western Blot Protocol: Download

