RBPMS is the marker of choice for RGC research
In recent years, RBPMS (RNA binding protein with multiple splicing) has emerged as the gold standard marker for identifying retinal ganglion cells (RGCs). For years, PhosphoSolutions’ (now part of Antibodies Inc.) 1832-RBPMS guinea pig polyclonal antibody has been the most widely cited choice among researchers, trusted for its specificity and performance.
We are excited to introduce an even better version of this essential tool—one that preserves all the unique properties researchers have come to rely upon in a new highly consistent recombinant format.
Limitations of traditional guinea pig polyclonal antibodies
Guinea pigs have long been valuable hosts for generating polyclonal antibodies thanks to their phylogenetic distance from other mammals and their distinct advantages in multiplexing—particularly when used alongside mouse or rabbit antibodies. However, traditional guinea pig polyclonals have faced limitations, most notably lower serum volumes and reduced antibody yields compared with larger species like rabbits, goats, or sheep.
A recombinant guinea pig antibody is a game changer
Our new recombinant guinea pig RBPMS antibody (cat. # 1834-RBPMS) is a chimeric antibody, combining the antigen-binding variable region of RBPMS mouse clone OTI3B7 with the constant region of the guinea pig IgG molecule. It retains the same binding performance as the original clone OTI3B7 but can be detected using standard anti-guinea pig secondary antibodies, making it perfect for reproducible multiplexing.
Creating a recombinant antibody eliminates the challenges of traditional guinea pig polyclonals while providing benefits that go well beyond multiplexing. Because recombinant antibodies are defined by an exact, unchanging sequence, they deliver truly consistent, reproducible performance—batch after batch, year after year with an inexhaustible source of antibody. This ensures you can generate dependable, repeatable data across experiments time and time again. Additionally, this antibody is produced in cultured cells under serum-free and protein-free conditions, making the production entirely animal-free.
Left: Immunostaining of mouse retinal section showing specific labeling of RGCs using our 1834-RBPMS antibody at 1:1000. Image kindly provided by Angela Wong, UCLA. Right: Western blotting of rat heart lysate with anti-RBPMS recombinant guinea pig monoclonal antibody at ~24 kD.
PhosphoSolutions continues its tradition of Antibodies that Work™
As a part of the Antibodies Inc. family, PhosphoSolutions continues its legacy of producing high-performance neuroscience antibodies with the release of our next-generation recombinant RBPMS antibody to the research community. We can’t wait to see the discoveries it helps you make!
We offer chicken recombinant antibodies as well
Chicken is another fantastic alternate species for multiplexing. Since birds are evolutionarily removed from mammals, chickens mount a robust immune response to mammalian proteins. They also offer lower background because chicken secondaries are less likely to cross-react with proteins in mammalian tissue samples.
Our lineup of chicken recombinant chimeric antibodies contains some of the top targets in neuroscience research, and all antibodies are offered in unconjugated, carrier free, and fluorophore conjugate formulations.