Products: Primary Antibodies

Anti-Synaptotagmin-3 Antibody (N278/19)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-269	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-269	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC WB

Target Description:

Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 3 is predominantly expressed in postsynaptic endocytic zones of neurons and has recently been shown to internalize AMPA receptors to depress synaptic strength and promote forgetting (Awasthi A. et al., 2019).

Immunogen:

Fusion protein amino acids 442-533 (cytoplasmic C2B domain) of mouse Synaptotagmin-3 (also known as Syt3, Synaptotagmin III and SytIII, accession number O35681)
Human: 100% identity (92/92 amino acids identical)
Rat: 98% identity (91/92 amino acids identical)
>70% identity with Synaptotagmin-9, Synaptotagmin-10 and Synaptotagmin-6

Cross Reactivity:

Does not cross-react with other Synaptotagmins

Protein Name(s):

Synaptotagmin-3 (Synaptotagmin III) (SytIII)

Gene Name(s):

Syt3

Antibody Registry ID:

Purified: AB_11001556
TC Supernatant: AB_11001828

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.