Products: Primary Antibodies

Anti-Shank3 (N69/46)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-109	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-109	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Synthetic peptide amino acids 840-857 (PEKLPGSLRKGIPRTKSV) of rat Shank3 (also known as SH3 and multiple ankyrin repeat domains protein 3, prolinerich synapse-associated protein 2, ProSAP2 and SPANK-2, accession number Q9JLU4)
Human: 100% identity (18/18 amino acids identical)
Mouse: 100% identity (18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity against Shank1 or Shank2

Protein Name(s):

SH3 and multiple ankyrin repeat domains protein 3 (Shank3) (Proline-rich synapse-associated protein 2) (ProSAP2) (SPANK-2)

Gene Name(s):

Shank3 Prosap2

Antibody Registry ID:

Purified: AB_2187730
TC Supernatant: AB_10698031

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

190 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Harony-Nicolas H,, Kay M, Hoffmann JD,, Klein ME, Bozdagi-Gunal O,, Riad M,, Daskalakis NP, Sonar S,, Castillo PE, Hof PR,,, Shapiro ML,, Baxter MG,, Wagner S, Buxbaum JD,,,,.. (2017), 'Oxytocin improves behavioral and electrophysiological deficits in a novel Shank3-deficient rat. .' Elife. 10.7554/eLife.18904.
Farley MM, Swulius MT, Waxham MN.. (2015), 'Electron tomographic structure and protein composition of isolated rat cerebellar, hippocampal and cortical postsynaptic densities. .' Neuroscience. 10.1016/j.neuroscience.2015.07.062.
Benthani F,, Tran PN, Currey N, Ng I, Giry-Laterriere M, Carey L, Kohonen-Corish MR,, Pangon L.. (2015), 'Proteogenomic Analysis Identifies a Novel Human SHANK3 Isoform. .' International Journal of Molecular Sciences. 10.3390/ijms160511522.
Braude JP, Vijayakumar S, Baumgarner K, Laurine R, Jones TA, Jones SM, Pyott SJ.. (), 'Deletion of Shank1 has minimal effects on the molecular composition and function of glutamatergic afferent postsynapses in the mouse inner ear. .' Hearing Research. 10.1016/j.heares.2015.01.008.
Duffney LJ, Wei J, Cheng J, Liu W, Smith KR, Kittler JT, Yan Z.. (2013), 'Shank3 deficiency induces NMDA receptor hypofunction via an actin-dependent mechanism. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1175-13.2013.
Durand CM, Perroy J, Loll F, Perrais D, Fagni L, Bourgeron T, Montcouquiol M, Sans N.. (2012), 'SHANK3 mutations identified in autism lead to modification of dendritic spine morphology via an actin-dependent mechanism. .' Molecular Psychiatry. 10.1038/mp.2011.57.
Bozdagi O#,, Sakurai T#,, Papapetrou D, Wang X, Dickstein DL, Takahashi N, Kajiwara Y, Yang M, Katz AM, Scattoni ML,, Harris MJ, Saxena R, Silverman JL, Crawley JN, Zhou Q,, Hof PR, Buxbaum JD,,.. (2010), 'Haploinsufficiency of the autism-associated Shank3 gene leads to deficits in synaptic function, social interaction, and social communication. .' Molecular Autism. 10.1186/2040-2392-1-15.
Ma XM, Kiraly DD, Gaier ED, Wang Y, Kim EJ, Levine ES, Eipper BA, Mains RE.. (2008), 'Kalirin-7 is required for synaptic structure and function. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4269-08.2008.