Products: Primary Antibodies

Anti-Shank3 Antibody (N69/46)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-109	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-109	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2b

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Target Description:

SH3 and multiple ankyrin repeat domains protein 3, or shank 3 (also known as proline-rich synapse-associated protein 2 (ProSAP2)) is a member of the shank family. Shank 3 binding domains include a SH3 domain, a proline rich domain, a PDZ domain and ankyrin repeat domains. Shank 3 is found in many tissues including heart and spleen, but is most abundantly expressed in brain at the post synaptic density. Shank 3 acts as a scaffold protein anchoring and connecting membrane bound receptors, ion channels and other proteins to the cytoskeleton in neurons to enable cell signaling. In this manner, Shank 3 may play a role in synapse formation and dendritic spine maturation. Mutations in the Shank 3 gene have been associated with neuropsychiatric diseases, including autism spectrm disorder.

Immunogen:

Synthetic peptide amino acids 840-857 (PEKLPGSLRKGIPRTKSV) of rat Shank3 (also known as SH3 and multiple ankyrin repeat domains protein 3, prolinerich synapse-associated protein 2, ProSAP2 and SPANK-2, accession number Q9JLU4); Human: 100% identity (18/18 amino acids identical); Mouse: 100% identity (18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity against Shank1 or Shank2

Protein Name(s):

Shank3

Gene Name(s):

Shank3 Prosap2

Antibody Registry ID:

Purified: AB_2187730
TC Supernatant: AB_10698031

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

190 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.