Products: Primary Antibodies

Anti-Mortalin/GRP75 (N52A/42)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-127	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-127	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Identified as off-target mAb in screen for anti-Salm2 NeuroMabs (immunogen was fusion protein amino acids 551-766 of mouse SALM2; see N52B/27 data sheet). Target identified as Mortalin/Grp75 by LC-MS/MS analysis of protein immunopurified from rat brain using N52A/42.

Cross Reactivity:

None Reported

Protein Name(s):

Stress-70 protein, mitochondrial (75 kDa glucose-regulated protein) (GRP-75) (Heat shock 70 kDa protein 9) (Mortalin) (Peptide-binding protein 74) (PBP74) (p66 MOT)

Gene Name(s):

Hspa9 Grp75 Hsp74 Hspa9a

Antibody Registry ID:

Purified: AB_2120479
TC Supernatant: AB_10674108

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

75 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

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Bishop HI, Guan D, Bocksteins E, Parajuli LK, Murray KD, Cobb MM, Misonou H, Zito K, Foehring RC, Trimmer JS.. (2015), 'Distinct Cell- and Layer-Specific Expression Patterns and Independent Regulation of Kv2 Channel Subtypes in Cortical Pyramidal Neurons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.1897-15.2015.
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Bishop HI, Cobb MM, Kirmiz M, Parajuli LK,, Mandikian D, Philp AM, Melnik M, Kuja-Panula J, Rauvala H, Shigemoto R, Murray KD,, Trimmer JS.. (2018), 'Kv2 Ion Channels Determine the Expression and Localization of the Associated AMIGO-1 Cell Adhesion Molecule in Adult Brain Neurons. .' Frontiers in Molecular Neuroscience. 10.3389/fnmol.2018.00001.
Thiffault I, Speca DJ, Austin DC, Cobb MM, Eum KS, Safina NP, Grote L, Farrow EG, Miller N, Soden S, Kingsmore SF, Trimmer JS, Saunders CJ, Sack JT.. (2015), 'A novel epileptic encephalopathy mutation in KCNB1 disrupts Kv2.1 ion selectivity, expression, and localization. .' The Journal of General Physiology. 10.1085/jgp.201511444.
Cobb MM, Austin DC, Sack JT, Trimmer JS.. (), 'Cell Cycle-dependent Changes in Localization and Phosphorylation of the Plasma Membrane Kv2.1 K+ Channel Impact Endoplasmic Reticulum Membrane Contact Sites in COS-1 Cells. .' The Journal of Biological Chemistry. 10.1074/jbc.M115.690198.
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Cerda O,,, Cáceres M,, Park KS,, Leiva-Salcedo E,, Romero A, Varela D,, Trimmer JS#,, Stutzin A#.. (2015), 'Casein kinase-mediated phosphorylation of serine 839 is necessary for basolateral localization of the Ca²⁺-activated non-selective cation channel TRPM4. .' Pflügers Archiv: European Journal of Physiology. 10.1007/s00424-014-1610-3.
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