Products: Primary Antibodies

Anti-MAP3K12 (N377/20)


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PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
75-355
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$205.00
Catalog #
73-355
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$180.00

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Immunogen:

Fusion protein amino acids 727-888 (C-terminus) of mouse MAP3K12 (also known as Mitogen activated protein kinase kinase kinase 12, Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mixed lineage kinase, DLK, ZPK and MUK, accession number Q60700) Rat: 97% identity (158/162 amino acids identical) Human: 86% identity (143/166 amino acids identical) <40% identity with MAP3K13

Cross Reactivity:

Does not cross-react with MAP3K13

Protein Name(s):

Mitogen-activated protein kinase kinase kinase 12 (EC 2.7.11.25) (Dual leucine zipper bearing kinase) (DLK) (Leucine-zipper protein kinase) (ZPK) (MAPK-upstream kinase) (MUK) (Mixed lineage kinase)

Gene Name(s):

Map3k12 Zpk

Antibody Registry ID:

Purified: AB_2315888
TC Supernatant: AB_2315887


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Expected Banding Pattern:

120 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.