Products: Primary Antibodies

Anti-Kir2.1 K+ channel Antibody (N112B/14)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-210	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-210	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Target Description:

Kir2.1 (KCNJ2) is a member of the inward rectifier potassium ion channel family which also includes Kir2.2 and Kir2.3. Kir2.1 is expressed in the membranes of many tissues, including heart, skeletal muscle, kidney and diffusely distributed throughout the brain. Kir2.1 has a role in cardiac excitability and has been shown to have interactions with CLG4, Interleukin 16 and TRAK2. Defects in Kir2.1 are involved with the diseases long QT syndrome type 7 (also known as andersen-Tawil syndrome), short QT syndrome 3, and Familial Atrial Fibrillation 9.

Immunogen:

Synthetic peptide amino acids 390-411 (cytoplasmic C-terminus) of mouse Kir2.1 also known as Inward rectifier potassium channel 2 or subfamily J member 2, Kcnj2 and Irk1, accession number P35561); Rat: 100% identity (22/22 amino acids identical); Human: 90% identity (20/22 amino acids identical)

Cross Reactivity:

No cross-reactivity against Kir2.2 or Kir2.3

Protein Name(s):

Kir2.1 K+ channel

Gene Name(s):

Kcnj2 Irk1

Antibody Registry ID:

Purified: AB_11000720
TC Supernatant: AB_11001668

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

55 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.