Products: Primary Antibodies

Anti-Kir2.1 K+ channel (N112B/14)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-210	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-210	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC WB

Immunogen:

Synthetic peptide amino acids 390-411 (cytoplasmic C-terminus) of mouse Kir2.1 also known as Inward rectifier potassium channel 2 or subfamily J member 2, Kcnj2 and Irk1, accession number P35561)
Rat: 100% identity (22/22 amino acids identical)
Human: 90% identity (20/22 amino acids identical)

Cross Reactivity:

No cross-reactivity against Kir2.2 or Kir2.3

Protein Name(s):

Inward rectifier potassium channel 2 (Inward rectifier K(+) channel Kir2.1) (IRK-1) (Potassium channel, inwardly rectifying subfamily J member 2)

Gene Name(s):

Kcnj2 Irk1

Antibody Registry ID:

Purified: AB_11000720
TC Supernatant: AB_11001668

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

55 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.

Citations and References

Lisewski U, Koehncke C, Wilck N, Buschmeyer B, Pieske B, Roepke TK.. (2016), 'Increased aldosterone-dependent Kv1.5 recycling predisposes to pacing-induced atrial fibrillation in Kcne3-/- mice. .' The FASEB Journal. 10.1096/fj.201600317R.
Zhang X,, Ai X,, Nakayama H, Chen B, Harris DM, Tang M, Xie Y, Szeto C, Li Y, Li Y,, Zhang H, Eckhart AD, Koch WJ, Molkentin JD, Chen X.. (2016), 'Persistent increases in Ca(2+) influx through Cav1.2 shortens action potential and causes Ca(2+) overload-induced afterdepolarizations and arrhythmias. .' Basic Research in Cardiology. 10.1007/s00395-015-0523-4.
El Gebeily G, El Khoury N, Mathieu S, Brouillette J, Fiset C.. (2015), 'Estrogen regulation of the transient outward K(+) current involves estrogen receptor α in mouse heart. .' Journal of Molecular and Cellular Cardiology. 10.1016/j.yjmcc.2015.07.013.
DiFranco M, Yu C, Quiñonez M, Vergara JL.. (2015), 'Inward rectifier potassium currents in mammalian skeletal muscle fibres. .' The Journal of Physiology. 10.1113/jphysiol.2014.283648.
Shy D, Gillet L, Ogrodnik J, Albesa M, Verkerk AO, Wolswinkel R, Rougier JS, Barc J, Essers MC, Syam N, Marsman RF, van Mil AM, Rotman S, Redon R, Bezzina CR, Remme CA, Abriel H.. (2014), 'PDZ domain-binding motif regulates cardiomyocyte compartment-specific NaV1.5 channel expression and function. .' Circulation. 10.1161/CIRCULATIONAHA.113.007852.
Luo X, Pan Z, Shan H, Xiao J, Sun X, Wang N, Lin H, Xiao L, Maguy A, Qi XY, Li Y, Gao X, Dong D, Zhang Y, Bai Y, Ai J, Sun L, Lu H, Luo XY, Wang Z, Lu Y, Yang B, Nattel S.. (2013), 'MicroRNA-26 governs profibrillatory inward-rectifier potassium current changes in atrial fibrillation. .' Journal of Clinical Investigation. 10.1172/JCI62185.
Milstein ML, Musa H, Balbuena DP, Anumonwo JM, Auerbach DS, Furspan PB, Hou L, Hu B, Schumacher SM, Vaidyanathan R, Martens JR, Jalife J.. (2012), 'Dynamic reciprocity of sodium and potassium channel expression in a macromolecular complex controls cardiac excitability and arrhythmia. .' Proceedings of the National Academy of Science of the United States of America. 10.1073/pnas.1109370109.
Cazorla M, Shegda M, Ramesh B, Harrison NL, Kellendonk C.. (2012), 'Striatal D2 receptors regulate dendritic morphology of medium spiny neurons via Kir2 channels. .' Journal of Neuroscience. 10.1523/JNEUROSCI.6056-11.2012.
Shen T, Aneas I, Sakabe N, Dirschinger RJ, Wang G, Smemo S, Westlund JM, Cheng H, Dalton N, Gu Y, Boogerd CJ, Cai CL, Peterson K, Chen J, Nobrega MA, Evans SM.. (2011), 'Tbx20 regulates a genetic program essential to adult mouse cardiomyocyte function. .' Journal of Clinical Investigation. 10.1172/JCI59472.
Cheng L, Yung A, Covarrubias M, Radice GL.. (2011), 'Cortactin is required for N-cadherin regulation of Kv1.5 channel function. .' The Journal of Biological Chemistry. 10.1074/jbc.M111.218560.
Farraj AK, Hazari MS, Haykal-Coates N, Lamb C, Winsett DW, Ge Y, Ledbetter AD, Carll AP, Bruno M, Ghio A, Costa DL.. (2011), 'ST depression, arrhythmia, vagal dominance, and reduced cardiac micro-RNA in particulate-exposed rats. .' Am J Respir Cell Mol Biol.. 10.1165/rcmb.2009-0456OC.