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Products: Primary Antibodies

Anti-KCC2 Antibody (N1/12)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-013
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-013
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

IHC IP WB

Target Description:

KCC2 or Potassium-chloride transporter member 5 is a member of the chloride potassium symporter family. KCC2 is a neuron-specific membrane protein encoded by the SLC12A5 gene in humans. The protein colocalizes with GABAA receptors and has for function to mediates electroneutral potassium-chloride cotransport in mature neurons to mainatin the Cl- homeostasis. KCC2 is widely expressed throughout the central nervous system, especially the hippocampus, hypothalamus, brainstem, and motoneurons of the ventral spinal cord. KCC2 is a neuronal marker that is often used to measure post-synaptic inhibition through GABAA receptors.

Immunogen:

Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2 (accession number Q63633); Human: 96% identity (108/112 residues identical); Mouse=95% identity (107/112 residues identical); Less than 20% identity with KCC1, KCC3, KCC4

Cross Reactivity:

No cross-reactivity reported

Protein Name(s):

KCC2

Gene Name(s):

Slc12a5 Kcc2 Kiaa1176

Antibody Registry ID:

Purified: AB_10672851
TC Supernatant: AB_10697875


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

140 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.