Human Mouse Rat
IHC IP WB
KCC2 or Potassium-chloride transporter member 5 is a member of the chloride potassium symporter family. KCC2 is a neuron-specific membrane protein encoded by the SLC12A5 gene in humans. The protein colocalizes with GABAA receptors and has for function to mediates electroneutral potassium-chloride cotransport in mature neurons to mainatin the Cl- homeostasis. KCC2 is widely expressed throughout the central nervous system, especially the hippocampus, hypothalamus, brainstem, and motoneurons of the ventral spinal cord. KCC2 is a neuronal marker that is often used to measure post-synaptic inhibition through GABAA receptors.
Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2 (accession number Q63633); Human: 96% identity (108/112 residues identical); Mouse=95% identity (107/112 residues identical); Less than 20% identity with KCC1, KCC3, KCC4
No cross-reactivity reported
Slc12a5 Kcc2 Kiaa1176
TC Supernatant: AB_10697875
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.