Products: Primary Antibodies

Anti-KCC2 (N1/12)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-013	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-013	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

IHC IP WB

Immunogen:

Fusion protein amino acids 932-1043 (intracellular carboxyl terminus) of rat KCC2 (accession number NP_599190)
Human: 96% identity (108/112 residues identical)
Mouse=95% identity (107/112 residues identical)
<20% identity with KCC1, KCC3, KCC4

Cross Reactivity:

None Reported

Protein Name(s):

Solute carrier family 12 member 5 (Electroneutral potassium-chloride cotransporter 2) (K-Cl cotransporter 2) (mKCC2) (Neuronal K-Cl cotransporter)

Gene Name(s):

Slc12a5 Kcc2 Kiaa1176

Antibody Registry ID:

Purified: AB_10672851
TC Supernatant: AB_10697875

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

140 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Campbell SL, Robel S, Cuddapah VA, Robert S, Buckingham SC, Kahle KT, Sontheimer H.. (2015), 'GABAergic disinhibition and impaired KCC2 cotransporter activity underlie tumor-associated epilepsy. .' Glia. 10.1002/glia.22730.
Markkanen M, Karhunen T, Llano O, Ludwig A, Rivera C, Uvarov P, Airaksinen MS.. (), 'Distribution of neuronal KCC2a and KCC2b isoforms in mouse CNS. .' Journal of Comparative Neurology. 10.1002/cne.23510.
He Q, Nomura T, Xu J, Contractor A.. (2014), 'The developmental switch in GABA polarity is delayed in fragile X mice. .' Journal of Neuroscience. 10.1523/JNEUROSCI.4447-13.2014.
Gagnon M, Bergeron MJ, Lavertu G, Castonguay A, Tripathy S, Bonin RP, Perez-Sanchez J, Boudreau D, Wang B, Dumas L, Valade I, Bachand K, Jacob-Wagner M, Tardif C, Kianicka I, Isenring P, Attardo G, Coull JA, De Koninck Y.. (2013), 'Chloride extrusion enhancers as novel therapeutics for neurological diseases. .' Nature Medicine. 10.1038/nm.3356.
Sun YG, Wu CS, Renger JJ, Uebele VN, Lu HC, Beierlein M.. (2012), 'GABAergic synaptic transmission triggers action potentials in thalamic reticular nucleus neurons. .' Journal of Neuroscience. 10.1523/JNEUROSCI.0839-12.2012.
Liu Q, Wong-Riley MT.. (), 'Postnatal development of Na(+)-K(+)-2Cl(-) co-transporter 1 and K(+)-Cl(-) co-transporter 2 immunoreactivity in multiple brain stem respiratory nuclei of the rat. .' Neuroscience. 10.1016/j.neuroscience.2012.03.018.
Döding A, Hartmann AM, Beyer T, Nothwang HG.. (), 'KCC2 transport activity requires the highly conserved L₆₇₅ in the C-terminal β1 strand. .' Biochemical and Biophysical Research Communications. 10.1016/j.bbrc.2012.02.147.
Horn Z, Ringstedt T, Blaesse P, Kaila K, Herlenius E.. (2010), 'Premature expression of KCC2 in embryonic mice perturbs neural development by an ion transport-independent mechanism. .' European Journal of Neuroscience. 10.1111/j.1460-9568.2010.07258.x.
Bragin DE, Sanderson JL, Peterson S, Connor JA, Müller WS.. (2009), 'Development of epileptiform excitability in the deep entorhinal cortex after status epilepticus. .' European Journal of Neuroscience. 10.1111/j.1460-9568.2009.06863.x.
Hedstrom KL, Ogawa Y, Rasband MN.. (2008), 'AnkyrinG is required for maintenance of the axon initial segment and neuronal polarity. .' The Journal of Biology. 10.1083/jcb.200806112.
Lee HH, Walker JA, Williams JR, Goodier RJ, Payne JA, Moss SJ.. (2007), 'Direct protein kinase C-dependent phosphorylation regulates the cell surface stability and activity of the potassium chloride cotransporter KCC2. .' The Journal of Biological Chemistry. 10.1074/jbc.M705053200.