Products: Primary Antibodies

Anti-GluN2C/NR2C (N422/18)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-411	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Catalog # 73-411	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot versus crude membrane fractions from GluN2C/NR2C knockout (KO) and wild-type (WT) mouse cerebella probed with N422/18 (top) and tubulin (bottom). Data courtesy of Bo-Shiun Chen (Augusta University) and reproduced with permission from Scientific Reports (2016 Chung et al, PMID 27845401).$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse Rat

Applications:

ICC IHC WB

Immunogen:

Fusion protein amino acids 836-1233 (cytoplasmic C-terminus) of rat GluN2C (also known as Glutamate receptor ionotropic NMDA 2C, Glutamate [NMDA] receptor subunit epsilon-3, Nmethyl D-aspartate receptor subtype 2C, NMDAR2C, NR2C and Grin2c, accession number Q00961)
Mouse: 93% identity (372/400 amino acids identical)
Human: 72% identity (293/405 amino acids identical)
30% identity with GluN2D/NR2D and other GluN/NMDA receptors

Protein Name(s):

Glutamate receptor ionotropic, NMDA 2C (GluN2C) (Glutamate [NMDA] receptor subunit epsilon-3) (N-methyl D-aspartate receptor subtype 2C) (NMDAR2C) (NR2C)

Gene Name(s):

Grin2c

Antibody Registry ID:

Purified: AB_2531892
TC Supernatant: AB_2491108

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine/immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

130 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.