ICC IHC WB
Gamma-aminobutyric acid receptor subunit alpha-4 or GABA AR alpha4 is encoded by the gene GABRA4. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 4 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha 4 has been found to be expressed in skeletal muscle tissue, adult mammalian kidney, heart and primarily in brain at the cell junction, synapse,and the postsynaptic cell membrane. Disorders involving the GABRA4 gene include autism, Status Epilepticus and restless legs syndrome.
Fusion protein amino acids 410-450 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha4 (also known as Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4 and Gabra4, accession number P28471)
Mouse: 90% identity (37/41 amino acids identical)
Human: 76% identity (30/39 amino acids identical)
50% identity with other GABA-A receptor Alpha subunits
Does not cross-react with other GABA-A receptor Alpha subunits
Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4)
TC Supernatant: AB_2336905
Antibody Validation and Application Notes
This antibody has been validated using the following assays:
This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.
The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.