Products: Primary Antibodies

Anti-GABA-A-R-Alpha4 Antibody (N398A/34)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-383	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $275.00
Brand	Catalog # 73-383	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $275.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:

ICC IHC WB

Target Description:

Gamma-aminobutyric acid receptor subunit alpha-4 or GABA AR alpha4 is encoded by the gene GABRA4. GABAA receptors are members of the Cys-loop family of ligand-gated ion channels. GABA AR alpha 4 is a subunit of the pentameric GABA receptors where GABA is the major inhibitory neurotransmitter in the vertebrate brain and mediates neuronal inhibition by binding to the GABA receptor and opening an integral chloride channel. GABA AR alpha 4 has been found to be expressed in skeletal muscle tissue, adult mammalian kidney, heart and primarily in brain at the cell junction, synapse,and the postsynaptic cell membrane. Disorders involving the GABRA4 gene include autism, Status Epilepticus and restless legs syndrome.

Immunogen:

Fusion protein amino acids 410-450 (cytoplasmic C-terminus) of rat GABA-A-R-Alpha4 (also known as Gamma-aminobutyric acid receptor subunit alpha-4, GABA(A) receptor subunit alpha-4 and Gabra4, accession number P28471)
Mouse: 90% identity (37/41 amino acids identical)
Human: 76% identity (30/39 amino acids identical)
50% identity with other GABA-A receptor Alpha subunits

Cross Reactivity:

Does not cross-react with other GABA-A receptor Alpha subunits

Protein Name(s):

Gamma-aminobutyric acid receptor subunit alpha-4 (GABA(A) receptor subunit alpha-4)

Gene Name(s):

Gabra4

Antibody Registry ID:

Purified: AB_2341108
TC Supernatant: AB_2336905

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:

This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

60 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.