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Products: Primary Antibodies

Anti-Doc2b Antibody (N150/21)

NeuroMab Logo from NeuroMab

 Datasheet

PRODUCT OPTIONS

Brand Catalog # Option Volume Concentration Price
Brand
NeuroMab Logo
Catalog #
75-214
Option
Purified
Volume
100 µL
Concentration
1 mg/mL
Price
$275.00
Brand
NeuroMab Logo
Catalog #
73-214
Option
TC Supernatant
Volume
5 mL
Concentration
Lot dependent
Price
$275.00

Validation Images


Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Mouse Rat

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC WB

Target Description:

Double C2-like domain-containing protein beta or Doc2b is encoded by the gene DOC2b. Doc2b functions as a calcium sensor which positively regulates soluble NSF attachment receptor (SNARE) dependent fusion of vesicles with membranes. Doc2b binds phospholipids and may act at the priming stage of membrane fusion by modifying membrane curvature to stimulate fusion. Doc2b is expressed in pancreatic cells, endocrine cells and brain, where it is highly expressed in neurons. (Friedrich et al.Neurosci., July 2, 2008 • 28(27):6794 – 6806)

Immunogen:

Fusion protein amino acids 1-216 (N-terminus) of rat Doc2b (also known as Double C2-like domain-containing protein beta and Doc2-beta, accession number P70610); Mouse: 97% identity (210/216 amino acids identical); Human: 93% identity (203/169 amino acids identical); Less than 60% identity with Doc2a

Cross Reactivity:

Does not cross-react with Doc2a

Protein Name(s):

Doc2b

Gene Name(s):

Doc2b

Antibody Registry ID:

Purified: AB_2315830
TC Supernatant: AB_2277497


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Immunohistochemistry:
This antibody shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.

Molecular Weight:

45 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected immunoperoxidase-diaminobenzidine / immunofluorescence staining pattern when used to stain brain sections.