Products: Primary Antibodies

Anti-DNAIC1 Antibody (UNC 65.56.18.11)

from NeuroMab

PRODUCT OPTIONS

Brand	Catalog #	Option	Volume	Concentration	Price
Brand	Catalog # 75-372	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $285.00
Brand	Catalog # 73-372	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $285.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Product Details

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse

Applications:

ICC WB

Target Description:

Dynein intermediate chain 1, axonemal or DNAIC1 is encoded by the gene DNAI1 and is a member of the dynein intermediate chain family. DNAI1 is an intermediate chain dynein in respiratory cilia. This protein has varied functions including development of respiratory function, motility of spermatozoa, and asymmetrical organization of the viscera during embryogenesis. Diseases associated with DNAI1 include Ciliary Dyskinesia and Kartagener Syndrome.

Immunogen:

Synthetic peptide amino acids 22-41 (RGTRKRDEDSGTEVGEGTDE) of human DNAIC1 (also known as Axonemal dynein intermediate chain 1 and Dnai1, accession number Q9UI46)
Mouse: 85% identity (17/20 amino acids identical)
Rat: 75% identity (15/20 amino acids identical) Hybridoma kindly donated by Larry Ostrowski, University of North Carolina at Chapel Hill School of Medicine. This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Protein Name(s):

Dynein intermediate chain 1, axonemal (Axonemal dynein intermediate chain 1)

Gene Name(s):

DNAI1

Antibody Registry ID:

Purified: AB_2341107
TC Supernatant: AB_2315828

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Molecular Weight:

80 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.