Products: Primary Antibodies

Anti-DNAIC1 (UNC 65.56.18.11)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-372	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-372	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Human Mouse

Applications:

ICC WB

Immunogen:

Synthetic peptide amino acids 22-41 (RGTRKRDEDSGTEVGEGTDE) of human DNAIC1 (also known as Axonemal dynein intermediate chain 1 and Dnai1, accession number Q9UI46)
Mouse: 85% identity (17/20 amino acids identical)
Rat: 75% identity (15/20 amino acids identical) Hybridoma kindly donated by Larry Ostrowski, University of North Carolina at Chapel Hill School of Medicine. This NeuroMab antibody is considered "Restricted" and is therefore not available for commercial re-distribution on a for-profit basis.

Protein Name(s):

Dynein intermediate chain 1, axonemal (Axonemal dynein intermediate chain 1)

Gene Name(s):

DNAI1

Antibody Registry ID:

Purified: AB_2341107
TC Supernatant: AB_2315828

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Knockout Validation:

This antibody has been knockout-validated in mouse brain (by Western blot and/or immunohistochemistry).

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

80 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it shows the expected staining pattern when used to stain COS cells overexpressing target.