Products: Primary Antibodies

Anti-BKbeta2 K+ channel (N53/32)

PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-087	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $205.00
Catalog # 73-087	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $180.00

* Prices shown reflect any applicable non-profit/academic discount. NeuroMab antibodies will have higher for-profit/distribution and orders placed online will need to be adjusted. Please inquire with us for more information.

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 20-60 ug/mL monoclonal antibody (mAb). Supernatant is suitable for most applications, including Western Blot, immunocytochemistry, immunohistochemistry, etc., and is a very economical choice compared to competitor offerings. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IP WB

Immunogen:

Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2 (accession number Q9CZM9)
Human: 97% (N-terminus) and 100% (C-terminus) identity (40/41 and 18/18 amino acids identical)
Mouse: 97% (N-terminus) and 100% (C-terminus) identity (40/41 and 18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4

Protein Name(s):

Calcium-activated potassium channel subunit beta-2 (BK channel subunit beta-2) (BKbeta2) (Calcium-activated potassium channel, subfamily M subunit beta-2) (Charybdotoxin receptor subunit beta-2) (K(VCA)beta-2) (Maxi K channel subunit beta-2) (Slo-beta-2)

Gene Name(s):

Kcnmb2

Antibody Registry ID:

Purified: AB_2131396
TC Supernatant: AB_10674434

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

27 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.