Products: Primary Antibodies

Anti-BKbeta2 K+ channel (N53/32)

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PRODUCT OPTIONS

Catalog #	Option	Volume	Concentration	Price*
Catalog # 75-087	Option Purified	Volume 100 µL	Concentration 1 mg/mL	Price $269.00
Catalog # 73-087	Option TC Supernatant	Volume 5 mL	Concentration Lot dependent	Price $269.00

"TC Supernatant" vs. "Purified"

TC Supernatant
Hybridoma tissue-culture supernatant (5mL) generally containing 10-50 ug/mL monoclonal antibody (clone-dependent) can be a very economical choice. Supernatant is suitable for most applications including Western Blot, immunocytochemistry, immunohistochemistry etc. For applications requiring higher antibody concentration or purity (such as immunoprecipitation, microinjection, or conjugation) it is recommended that you purchase the purified form of the antibody (supplied at 1mg/mL).

Purified mAbs
Produced by in vitro bioreactor culture of hybridoma lines followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody at 1mg/mL.

Validation Images

$Immunoblot against crude membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N53/32 (left) or N52A/42 (right) TC supe.$

Overview

Brand:

NeuroMab

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG2a

Species Reactivity:

Human Mouse Rat

Applications:

ICC IP WB

Immunogen:

Fusion protein amino acids 1-41 (N-terminus) and 218-235 (C-terminus) of mouse BKBeta2 (accession number Q9CZM9)
Human: 97% (N-terminus) and 100% (C-terminus) identity (40/41 and 18/18 amino acids identical)
Mouse: 97% (N-terminus) and 100% (C-terminus) identity (40/41 and 18/18 amino acids identical)

Cross Reactivity:

No cross-reactivity against BKBeta1, BKBeta3 or BKBeta4

Protein Name(s):

Calcium-activated potassium channel subunit beta-2 (BK channel subunit beta-2) (BKbeta2) (Calcium-activated potassium channel, subfamily M subunit beta-2) (Charybdotoxin receptor subunit beta-2) (K(VCA)beta-2) (Maxi K channel subunit beta-2) (Slo-beta-2)

Gene Name(s):

Kcnmb2

Antibody Registry ID:

Purified: AB_2131396
TC Supernatant: AB_10674434

Antibody Validation and Application Notes

This antibody has been validated using the following assays:

Western Blot:

This antibody recognizes a single immunoreactive band of expected molecular weight when used to probe lysate from COS cells overexpressing target.

Immunocytochemistry:

This antibody shows the expected staining pattern when used to stain COS cells overexpressing target.

Expected Banding Pattern:

27 kDa

Quality Control

The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.

Citations and References

Chen L, Bi D, Tian L, McClafferty H, Steeb F, Ruth P, Knaus HG, Shipston MJ.. (2013), 'Palmitoylation of the β4-subunit regulates surface expression of large conductance calcium-activated potassium channel splice variants. .' The Journal of Biological Chemistry. 10.1074/jbc.M113.461830.
Zhang XL, Mok LP, Lee KY, Charbonnet M, Gold MS.. (2012), 'Inflammation-induced changes in BK(Ca) currents in cutaneous dorsal root ganglion neurons from the adult rat. .' Molecular Pain. 10.1186/1744-8069-8-37.
Shruti S, Urban-Ciecko J, Fitzpatrick JA, Brenner R, Bruchez MP, Barth AL.. (2012), 'The brain-specific Beta4 subunit downregulates BK channel cell surface expression. .' PLoS One. 10.1371/journal.pone.0033429.