Anti-VDAC1 Antibody (N152B/23)

Name: Anti-VDAC1 Antibody
Brand: NeuroMab
SKU: 75-204
Price: 337.0 USD
Availability: InStock

Our mouse VDAC1 monoclonal antibody from NeuroMab is produced in-house from clone N152B/23. It detects human, mouse, and rat VDAC1 and is purified by Protein A chromatography. Works in WB, IHC, ICC, ELISA.

Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).

Array tomography immunofluorescence of 70 nm sections of LRWhite-embedded adult mouse hippocampus. Two consecutive sections labeled with N152B/23 (bottom) and DAPI (top), where low level autofluorescence reveals mitochondria appearing as brighter elongated structures within darker apical dendrites (arrows). Images courtesy of Kristina Micheva (Stanford).

Adult rat hippocampus immunohistochemistry.

Western blot for mitochondrial Ca 2+ uptake proteins in hippocampus,cortex and cerebellum lysates from 6 month old contol mice (Control) and mice with haploinsufficiency of the mitochondrial Ca2+ uniporter (MCU+/−). Image from publication CC-BY-4.0. PMID:36274588

Immunoblot against membranes from livers of wild-type (WT) and VDAC1 knockout (KO) mice and brains of WT mice (MBM) and rat (RBM). Liver samples courtesy of Tatiana Sheiko and Bill Craigen (Baylor College of Medicine).

Click on image to zoom

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Biotin

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-204

Volume: 100 µL

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

VDAC1

Scientific Background

Voltage-dependent anion-selective channel protein 1 or Outer Mitochondrial Membrane Protein Porin 1 is encoded by the gene VDAC1 and belongs to the mitochondrial porin family. VDAC1 is a voltage-dependent anion channel protein that is a major component of the outer mitochondrial membrane and is also found on the plasma membrane of cells. VDAC1 can act as a scaffold for many proteins and allows for the passage of ions and metabolites, including ATP, through interactions within the pore. VDAC1 in the plasma membrane plays a role in the regulation of cell volume and apoptosis. VDAC1 is expressed in several tissues including heart, brain, liver and skeletal muscle. Diseases associated with VDAC1 include Influenza and Typhoid Fever.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N152B/23

Isotype

IgG2a

Applications

ELISA, ICC, IHC, WB

Host Species

Mouse

Gene Name

VDAC1 VDAC

Molecular Weight

30 kDa

Immunogen

Fusion protein amino acids 1-283 (full-length) of human VDAC1 (accession number P21796) produced recombinantly in E. Coli

Immunogen Species

Human

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2214807

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125

Dilution Ranges

WB: 1:1000
IHC: 1:250
ICC: 1:500

Conjugate

Unconjugated

Specificity

Does not cross-react with VDAC2 or VDAC3 (based on KO validation results)

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Synonyms

PORIN; Voltage-dependent anion-selective channel protein 1; outer mitochondrial membrane protein porin 1; VDAC-1; plasmalemmal porin; hVDAC1; porin 31HL; porin 31HM; sperm binding protein 1a

UniProt ID

P21796
UniProt Summary: Non-selective voltage-gated ion channel that mediates the transport of anions and cations through the mitochondrion outer membrane and plasma membrane. The channel at the outer mitochondrial membrane allows diffusion of small hydrophilic molecules; in the plasma membrane it is involved in cell volume regulation and apoptosis. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV. The open state has a weak anion selectivity whereas the closed state is cation-selective. Binds various signaling molecules, including the sphingolipid ceramide, the phospholipid phosphatidylcholine, and the sterols cholesterol and oxysterol. In depolarized mitochondria, acts downstream of PRKN and PINK1 to promote mitophagy or prevent apoptosis; polyubiquitination by PRKN promotes mitophagy, while monoubiquitination by PRKN decreases mitochondrial calcium influx which ultimately inhibits apoptosis. May participate in the formation of the permeability transition pore complex (PTPC) responsible for the release of mitochondrial products that triggers apoptosis. May mediate ATP export from cells. Part of a complex composed of HSPA9, ITPR1 and VDAC1 that regulates mitochondrial calcium-dependent apoptosis by facilitating calcium transport from the ER lumen to the mitochondria intermembrane space thus providing calcium for the downstream calcium channel MCU that directly releases it into mitochondria matrix. Mediates cytochrome c efflux.

NCBI Gene ID

7416

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Anti-VDAC1 Antibody (N152B/23)

SKU: 75-204

Product Details

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