Anti-Tyrosine Kinase Receptor A (TrkA) Antibody (BS470)

Our Anti-Tyrosine Kinase Receptor A (TrkA) mouse monoclonal primary antibody detects human Tyrosine Kinase Receptor A (TrkA), and is IgG. It is validated for use in FC, ICC.

TrkA expression on non-permeabilized human SHSY-5Y neuroblastoma and DU145 prostate cancer cell lines analysed by Flow Cytometry. Blocking: 200 µg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat # M-1723-100, 2 μg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow™ instrument and protocols.
Immunofluorescence analysis of TrkA expression on human SHSY-5Y neuroblastoma cell membrane. Fixed (4% formaldehyde), non-permeabilized, and blocked (10% normal horse serum) SHSY-5Y cells were incubated with TrkA antibody M-1723-100 (extracellular domain, 2 µg/mL, green) for 1 hour. Primary antibody binding was visualized with a secondary donkey anti-mouse-CF488A antibody (4 µg/mL, 1 hour incubation). Cell nuclei were stained with Hoechst dye (blue). Magnification: 100x.
Comparison of TrkA expression on non-permeabilized and permeabilized human SHSY-5Y neuroblastoma cells by Flow Cytometry. This batch of SHSY-5Y cells did not show TrkA expression on the membrane (left image), but immunoreactivity in permeabilized cells (right image), suggesting that TrkA is stored intracellularly. Conditions: Permeabilization with absolute methanol (10 minutes on ice), or no permeabilization (incubation of primary antibody on ice only). Blocking: 200 µg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat# M-1723-100, 2 µg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
TrkA expression on non-permeabilized human SHSY-5Y neuroblastoma and DU145 prostate cancer cell lines analysed by Flow Cytometry. Blocking: 200 µg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat # M-1723-100, 2 μg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow™ instrument and protocols.
TrkA expression on non-permeabilized human SHSY-5Y neuroblastoma and DU145 prostate cancer cell lines analysed by Flow Cytometry. Blocking: 200 µg/mL normal sheep IgG (30 minutes) on ice. Primary antibody: Mouse Monoclonal antibody to TrkA, extracellular domain (cat # M-1723-100, 2 μg per ~106 cells) for 30 minutes on ice. Secondary antibody: Goat anti-mouse PE (1:100 dilution, 20 min in dark on ice. Non-specific Control IgG, clone X63 (cat# M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo Moxiflow™ instrument and protocols.
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Human
Flow, ICC
Mouse

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Data Sheet

SKU: M-1723-100

Volume/Size: 100 µg
Price:
$327.00

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