Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N275/14 (left) and N86/8 (right).
Synaptotagmin-7 (Synaptotagmin VII) (SytVII)
Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Synaptotagmin 7 is highly, ubiquitously expressed in the brain and was originally identified as a Ca2+ sensor for lysosomal exocytosis, but has since been demonstrated to play roles in secretion from many different cell types (MacDougall DD, et al., 2018)
ICC, IHC, WB
45 kDa (plus others)
Fusion protein amino acids 150-239 (cytoplasmic C2A domain) of mouse Synaptotagmin-7 (also known as Syt7, Synaptotagmin VII and SytVII, accession number Q9R0N7)
Rat: 98% identity (89/90 amino acids identical)
Human: 97% identity (88/90 amino acids identical)
Greater than 50% identity with Synaptotagmin-6 (and other Synaptotagmins)
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.