Transfected cell immunoblot: extracts of COS cells transiently transfected with GFP-tagged Synaptotagmin or untagged Kv2.1 plasmids and probed with N274/8 (left) and N86/8 (right).
Bulk Order Anti-Synaptotagmin-5 Antibody
Synaptotagmins are a family of calcium sensing, membrane trafficking proteins. 17 different synaptotagmins have been identified in mammals. Along with synaptotagmins 1 and 2, synaptotagmin 5 (also known as synaptotagmin 9) has been shown to function as a Ca(2+) sensor for fast synchronous neurotransmitter release (Xu J et al., 2007).
Purified by Protein A chromatography
ICC, IHC, WB
Fusion protein amino acids 236-323 (cytoplasmic C2A domain) of mouse Synaptotagmin-5 (accession number Q9R0N9) produced recombinantly in E. Coli
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Does not cross-react with Synaptotagmin-10 (or other Synaptotagmins)
Each new lot of antibody is quality control tested on cells overexpressing target protein and confirmed to give the expected staining pattern.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.