Anti-Rhodopsin Antibody (B630)

Name: Anti-Rhodopsin Antibody
Brand: Biosensis
SKU: M-1815-100
Price: 327.0 USD
Availability: InStock

Our Anti-Rhodopsin mouse monoclonal primary antibody detects bovine, human, mouse, pig, and rat Rhodopsin, and is affinity-purified. It is validated for use in IHC-Frozen, WB.

Left: Pig retinal section stained for rhodopsin by Immunohistochemistry with mouse antibody to Rhodopsin (green). Rhodopsin is most abundant in the rod outer segments (ROS) of retina, clearly localized in rod membranes. The rod inner segments (RIS) and rod nuclei in the outer nuclear layer (ONL) are also seen in this image. Nuclear DNA was stained with DAPI (blue). Right: Western Blot of bovine retinal extract probed with mouse antibody to Rhodopsin. The antibody stains a band corresponding to retinal rhodopsin at about 35 kDa. Higher molecular weight bands represent aggregated forms of rhodopsin.

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Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Applications

IHC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1815-100

Volume/Size: 100 µg

Variant

Price:

$327.00

Quantity:

Product Details

Target

Rhodopsin

Scientific Background

Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth (By similarity). Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change that activates signaling via G-proteins (PubMed:10926528, PubMed:12044163, PubMed:11972040, PubMed:16908857, PubMed:16586416, PubMed:17060607, PubMed:17449675, PubMed:18818650, PubMed:21389983, PubMed:22198838, PubMed:23579341, PubMed:25205354, PubMed:27458239). Subsequent receptor phosphorylation mediates displacement of the bound G-protein alpha subunit by the arrestin SAG and terminates signaling (PubMed:1396673, PubMed:15111114). Ref: uniprot.org

Format

Affinity-purified

Clonality

Monoclonal

Clone

B630

Isotype

IgG1

Applications

IHC, WB

Host Species

Mouse

Immunogen

Purified bovine rhodopsin

Immunogen Species

Bovine

Species Reactivity

Bovine, Human, Mouse, Pig, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C. After reconstitution divide into aliquots and store at -20°C for long-term storage. Store at 2-8°C short-term (up to 4 weeks) with an appropriate antibacterial agent. Avoid repetitive freeze/thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 0.1% trehalose, and sodium azide.

Dilution Ranges

WB: 1:5000
IHC: 1:1000

Application Details

Due to the highly hydrophobic nature of rhodopsin, avoid boiling samples in SDS-PAGE sample buffer for rhodopsin analysis by Western Blotting, as this will result in extensive aggregation of the rhodopsin protein and appearance of high molecular weight bands.

Conjugate

Unconjugated

Specificity

Bovine, reacts with human, rat, mouse, cow, pig. Expected to react with other mammalian species.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

UniProt ID

P02699

Shipping

25°C (ambient)

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Anti-Rhodopsin Antibody (B630)

SKU: M-1815-100

Product Details

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