Our Anti-nNOS/NOS1 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L121/25. It detects human, mouse, and rat nNOS/NOS1, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.
Array tomography immunofluorescence volume reconstruction of 9 serial LRWhite-embedded 70 nm sections from adult mouse cortex layer 6 with L121/25 (green), rabbit Synapsin (Cell Signaling #5297, red). Image courtesy of Kristina Micheva (Stanford).
Nitric Oxide Synthase 1 is encoded by the gene NOS1. NOS1 is a member of the NOS family. NOS1 synthesizes nitric oxide from L-arginine, which acts as a biologic mediator in several processes, including neurotransmission, and antimicrobial and antitumoral activities. NOS1 is expressed ubiquitously, with high levels of expression in skeletal muscle. Diseases associated with NOS1 include Achalasia, Familial Esophageal and Pyloric Stenosis, Infantile Hypertrophic, 1.
Purified by Protein A chromatography
ICC, IHC, WB
155-165 kDa for full-length protein, smaller fragments possible due to proteolysis and/or variable sample preparation
Fusion protein amino acids 350-720 of human NOS1 (accession number P29475) produced recombinantly in E. Coli
Human, Mouse, Rat
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
No cross-reactivity reported
Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.