Adult mouse brain membrane (MBM) and transfected cell immunoblot: extracts of MBM and COS cells transiently transfected with Neuroligin-1 and Neuroligin-4* plasmids and probed with N98/7. Data courtesy of Marc Bolliger and Tom Sudhof, UTSW (now at Stanford).
Neuroligin4 is encoded by the gene NLGN4. Neuroligin4 is a transmembrane scaffolding protein and cell adhesion molecule. It is involved in cell-cell interactions in neurons and in other cell types. It is expressed in many tissues, including retina and brain, where it is located at the postysynaptic membrane and is involved in regulation of synapses, synapse formation and synaptic transmission. Diseases associated with this gene include forms of Autism and Asperger Syndrome.
Nlgn4l Nl4* Nlgn4 Nlgn4x
Fusion protein amino acids 782-945 (intracellular C-terminus) of mouse Neuroligin-4* (accession number ABS19580)
~45% identity with Neuroligin-1, -2 and -3
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Does not cross-react with other Neuroligins
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.