Stable/transfected cell immunoblot: extracts of HEK cells stably expressing Nav1.7 and COS cells transiently transfected with GFP-tagged Nav1.8 or untagged Kv2.1 plasmids and probed with N134/12 TC supe.
Anti-Nav1.8 Na+ Channel Antibody
Nav1.8 Na+ channel
NAV1.8 is a member of the voltage-gated sodium channel family, which function in producing and propagating action potentials. Sodium channels are composed of a large alpha subunit that may be associated with one or more smaller beta subunits. Nav 1.8 is an integral membrane glycoprotein that is responsible for the initial rising phase of action in most excitable cells. Nav 1.8 is expressed in neurons in the dorsal root ganglion (DRG). and appears to have a role in pain pathways.
ICC, IHC, WB
Fusion protein amino acids 1724-1956 (cytoplasmic C-terminus) of rat Nav1.8(also known as Sodium channel protein type 10 or X subunit alpha, Voltage-gated sodium channel subunit alpha Nav1.8, Peripheral nerve sodium channel 3, PN3 or Sensory neuron sodium channel, accession number Q62968); Mouse: 93% identity (220/235 amino acids identical); Human: 85% identity (200/234 amino acids identical); Greater than 50% identity with Nav1.7 and Nav1.5
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.