Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/33)

Name: Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody
Brand: NeuroMab
SKU: 75-116
Price: 319.0 USD
Availability: InStock

Our Anti-mGluR1/5 (Group I) glutamate receptor mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N75/33. It detects human, mouse, and rat mGluR1/5 (Group I) glutamate receptor, and is purified by Protein A chromatography. It is great for use in IHC, ICC, IP, WB.

Immunoblots on membranes prepared from whole mouse (MBM) and rat (RBM) brain, and from human cerebellum [HBM(Cb)], cerebral cortex [HBM(Cx)], and hippocampus [HBM(H)].

Adult rat hippocampus immunohistochemistry.

Electron micrograph shows N75/33 immunolabelling in the stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunogold particles were observed at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts (Den) of pyramidal cells establishing synaptic contacts with excitatory axon terminals (b). 80.000 X. Courtesy Dr. Rafael Lujan (Universidad de Castilla-La Mancha).

Adult rat brain membrane immunoblot.

Species Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Available Conjugates

490

550

594

650

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Data Sheet

SKU: 75-116

Volume: 100 µL

Variant

Price:

$319

Quantity:

Product Details

Target

mGluR1/5 (Group I) glutamate receptor

Target Description

The metabotropic glutamate receptors (mGluRs) are key receptors in the modulation of excitatory synaptic transmission in the central nervous system. They are implicated in many forms of neural plasticity as well as learning and memory and drug abuse (Bhattacharya et al., 2004; Francesconi et al., 2004; Wilson and Nicoll, 2001). Group I metabotropic glutamate receptors (consisting of mGluR1 and mGluR5) are G-protein-coupled neurotransmitter receptors that are localized in the perisynaptic region of the postsynaptic membrane. When activated, Group I mGluRs lead to stimulation of phospholipase and activation of Protein Kinase C. In contrast, activation of Group II metabotropic receptors (mGluR2 and mGluR3) leads to inhibition of adenylate cyclase. The mGluR1 receptor may also be critically involved in limiting the deleterious effects of excitotoxicity (Blaabjerg et al., 2003). In contrast, the mGluR5 receptor appears to be essential for late phase LTP in area CA1 of the hippocampus (Francesconi et al., 2004).

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N75/33

Isotype

IgG1

Applications

ICC, IHC, IP, WB

Host Species

Mouse

Gene Name

Grm5 Gprc1e Mglur5

Molecular Weight

130 kDa (both mGluR1 and mGluR5)

Antigen

Fusion protein amino acids 824-1203 (cytoplasmic C-terminus) of rat mGluR5b (accession number P31424) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse, Rat

Antibody Registry ID

AB_2232873

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.44

Conjugate

Unconjugated

Specificity

Recognizes mGluR1 and mGluR5

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Metabotropic glutamate receptor 5 (mGluR5)

UniProt Details

UniProt (Human): P41594
UniProt (Immunogen Species): P31424

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Anti-mGluR1/5 (Group I) Glutamate Receptor Antibody (N75/33)

SKU: 75-116

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