Anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Antibody

Name: Anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Antibody
Brand: Biosensis
SKU: GP-030-50
Price: 337.0 USD
Availability: InStock

Our Anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) guinea pig polyclonal primary antibody detects human, rat, and sheep Melanocyte-stimulating hormone, alpha (Alpha-MSH), and is whole serum. It is validated for use in IHC-Frozen.

Figure shows staining of cells in sheep hypothalamus using Guinea pig polyclonal antibody to human alpha MSH (GP-030-50) at 1:1000 followed by incubation with FITC labeled goat anti-guinea pig secondary antibodies.

Anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Antibody

Immunohistochemical identification of alpha MSH-immunoreactive neurons in cryostat sections from the hypothalamic arcuate nucleus of the rat. The signal was detected with the gp-030-50 primary antiserum (1:40,000), followed by its visualization with the biotinylated secondary antibody-ABC method and nickel-diaminobenzidine chromogen. Photo courtesy of Dr. Erik Hrabovszky, Hungarian Academy of Sciences, Budapest, Hungary.

Figure shows staining of cells in sheep hypothalamus using Guinea pig polyclonal antibody to human alpha MSH (GP-030-50) at 1:1000 followed by incubation with FITC labeled goat anti-guinea pig secondary antibodies.

Click on image to zoom

Species Reactivity

Human, Rat, Sheep

Applications

IHC

Host Species

Guinea Pig

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Data Sheet

SKU: GP-030-50

Volume/Size: 50 µL

Variant

Price:

$337.00

Quantity:

Product Details

Target

Melanocyte-stimulating hormone, alpha (Alpha-MSH)

Scientific Background

Proopiomelanocortin (POMC) is the precursor of the melanocortin peptides alpha, beta and gamma. POMC neurons in the arcuate nucleus of the hypothalamus function as key central regulators of food intake and homeostatic control of energy balance in both rodents and non-human primates. MSH increases the pigmentation of skin by increasing the melanin production in melanocytes. MSH is produced in the pituitary gland and belongs to the POMC family.

Format

Whole serum

Clonality

Polyclonal

Isotype

Mixed

Applications

IHC

Host Species

Guinea Pig

Immunogen

A synthetic peptide (Ac-SYSMEHFRWGKPV) corresponding to the amino acids 138-150 from human Proopiomelanocortin (POMC). POMC is the precursor of the melanocortin peptides alpha, beta and gamma. The synthetic peptide was conjugated to a carrier protein to enhance the immunological response.

Immunogen Species

Human

Species Reactivity

Human, Rat, Sheep

Storage

Spin vial briefly before opening. Reconstitute in 50 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized antibody at 2-8°C or lower. After reconstitution, divide into aliquots and store at -20°C, or lower, for optimal long term stability. It is recommended that a reconstituted aliquot is stored at 2-8°C for no longer than 2 weeks. Allocation of an appropriate anti-bacterial agent can increase shelf life by several weeks. Glycerol (1:1) can be added to neat serum for additional stability if intended use does not prevent this.

Physical State

Lyophilized

Production Notes

Neat serum

Dilution Ranges

IHC: 1:2000

Application Details

IHC performed in sheep brain (hypothalamus) demonstrates intense staining of cells. No staining is evident when the primary antibody is pre-absorbed with 0.5 mg/mL of alpha MSH.

Conjugate

Unconjugated

Specificity

Specificity was demonstrated by immunohistochemistry. This antibody is known to react with human, rat and sheep. Other species have not yet been tested.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (lyophilized, unopened vial).

Synonyms

Proopiomelanocortin; POMC; alpha-melanocyte stimulating hormone; melanotropin alpha

UniProt ID

P01189

Shipping

25°C (ambient)

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Anti-Melanocyte-stimulating hormone, alpha (Alpha-MSH) Antibody

SKU: GP-030-50

Product Details

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