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Products: Primary Antibodies

Anti-Tyrosine Hydroxylase (LNC1)

PRODUCT OPTIONS

Catalog # Option Volume Concentration Price*
Catalog #
76-700-100
Option
Ascites Fluid
Volume
100 µL
Concentration
1 mg/mL
Price
$329.00
Catalog #
76-700-020
Option
Ascites Fluid Trial Size
Volume
20 µL
Concentration
1 mg/mL
Price
$99.00

Validation Images


Overview

Brand:

Antibodies Incorporated

Antibody Type:

Monoclonal

Host Species:

Mouse

Antibody Isotype:

IgG1

Species Reactivity:

Chicken Frog Human Lizard Monkey Mouse Rat Vole Zebrafish

Applications:
WB Western Blot
IHC Immunohistochemistry
ICC Immunocytochemistry
IP Immunoprecipitation
AT Array Tomography
EM Electron Microscopy
CHIP Chromatin Immunoprecipitation
DB Dot Blot

ICC IHC IP WB

Immunogen:

Tyrosine Hydroxylase purified from PC12 cells.

LNC1 is an antibody which can be used to detect Tyrosine Hydroxylase (TH). TH is the rate-limiting enzyme in the synthesis of the catecholamine neurotransmitters dopamine, epinephrine, and norepinephrine and is responsible for converting L-tyrosine to L-dopa. Synthesis of catecholamines is regulated by the interaction of TH with its’ cofactor, tetrahydrobiopterin (BH4) and the substrates L-tyrosine and molecular oxygen. In humans four TH mRNA splice variants (hTH1-hTH4) have been isolated while subprimate species rely on a single form of TH.  It is known that the hTH1-hTH4 variants are identical in their catalytic domain but differ in their N-terminal regulatory domains. Importantly, LNC1 reacts with the catalytic domain of TH and thus with all four isoforms of human TH. The role of TH in the synthesis of catecholamine neurotransmitters suggests a connection between the enzyme and a number of neuropathogenic diseases characterized by irregular catecholamine levels, such as Parkinson's disease, schizophrenia, and dystonia, as well as a variety of cardiovascular diseases.

Protein Name(s):

Tyrosine 3-monooxygenase

Gene Name(s):

TH, TYH, EC 1.14.16.2


Antibody Validation and Application Notes


This antibody has been validated using the following assays:
Western Blot:

This antibody recognizes a single immunoreactive band of expected molecularweight when used to probe brain lysate.

Immunohistochemistry:
Fluorescent immunohistochemistry of sections from human midbrain showed the expected staining of dopaminergic neurons.

Molecular Weight:

59-63 kDa


Quality Control


The following quality control assay is performed on each new lot of this antibody to ensure it meets designated performance requirements.

Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.


Citations and References