Anti-Lamin A/C Antibody (4C4)

Name: Anti-Lamin A/C Antibody
Brand: Biosensis
SKU: M-1689-100
Price: 337.0 USD
Availability: InStock

Our Anti-Lamin A/C mouse monoclonal primary antibody detects human Lamin A/C, and is IgG. It is validated for use in FC, ICC, WB.

Left: HeLa cells stained with M-1689-100 (red), and counterstained with chicken polyclonal antibody to Vimentin (green, C-1409-50). The The Lamin A/C antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Right: Analysis of Lamin A/C expression in human prostate cancer DU145 cell line by FLow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Lamin A/C (cat # M-1689-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

Left: Lamin A/C expression in HeLa cells, stained with mouse antibody to lamin A/C (red, 1:2,000) by Immunocytochemistry. Co-staining was performed with a rabbit anti-HSP60 antibody (green). Blue: Hoechst nuclear stain. The lamin A/C antibody specifically labels the nuclear lamina. Right: Western blot analysis of lamin A/C expression in cell lysates using mouse anti-lamin A/C antibody (green, 1:1,000). [1] protein standard, [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65 kDa correspond to the lamin A and lamin C proteins, respectively, detected only in the cells of human origin. The lamin A/C antibody does not recognize rat or mouse proteins.

Left: HeLa cells stained with M-1689-100 (red), and counterstained with chicken polyclonal antibody to Vimentin (green, C-1409-50). The The Lamin A/C antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Right: Analysis of Lamin A/C expression in human prostate cancer DU145 cell line by FLow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Lamin A/C (cat # M-1689-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.

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Species Reactivity

Human

Applications

Flow, ICC, WB

Host Species

Mouse

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Data Sheet

SKU: M-1689-100

Volume/Size: 100 µg

Variant

Price:

$337.00

Quantity:

Ships: 1-2 business days

Product Details

Target

Lamin A/C

Scientific Background

The Lamin proteins are members of the intermediate filament protein family but are located inside the nucleus rather than in the cytoplasm (1). The lamins function as skeletal components tightly associated with the inner nuclear membrane. Originally the proteins of the nuclear cytoskeleton were named Lamin A, B and C, from top to bottom as visualized on SDS-PAGE gels. Subsequently it was found that Lamins A and C were coded for by a single gene (2), while the Lamin B band may contain two proteins encoded by two genes now called Lamin B1 and Lamin B2. Lamin A has a mass of about 74 kDa while Lamin C is 65 kDa. The Lamin A protein includes 98 amino acids missing from Lamin C, while Lamin C has a C-terminal 6 amino acid peptide not present in Lamin A. Apart from these regions Lamin A and C are identical so that antibodies raised against either protein are likely to cross react with the other, as is the case with this monoclonal. Lamin polymerization and depolymerization is regulated by phosphorylation by cyclin dependent protein kinase 1 (CDK1), the key component of "maturation promoting factor", the central regulator of cell division. Activity of this kinase increases during cell division and is responsible for the breakdown of the nuclear lamina. Mutations in the LMNA gene are associated with several serious human diseases, including Emery-Dreifuss muscular dystrophy, familial partial lipodystrophy, limb girdle muscular dystrophy, dilated cardiomyopathy, Charcot-Marie-Tooth disease type 2B1, and Hutchinson-Gilford progeria syndrome. This family of diseases belong to a larger group which are often referred to as Laminopathies, though some laminopathies are associated in defects in Lamin B1, B2 or one or other of the numerous nuclear lamina binding proteins. A truncated version of lamin A, commonly known as progerin, causes Hutchinson-Gilford progeria syndrome, a form of premature aging (3).

Format

IgG

Clonality

Monoclonal

Clone

4C4

Isotype

IgG1

Applications

Flow, ICC, WB

Host Species

Mouse

Immunogen

Full length recombinant human Lamin C

Immunogen Species

Human

Species Reactivity

Human

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water to achieve a 1 mg/mL concentration. Centrifuge to remove any insoluble material. After reconstitution of lyophilized antibody, aliquot and store at -20°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Production Notes

Protein G purified

Buffer

Lyophilized from PBS buffer pH 7.2-7.6 with 5% trehalose, and sodium azide

Dilution Ranges

WB: 1:1000-1:2000
ICC: 1:500-1:1000

Application Details

Use 2 ug/10^6 cells for Flow cytometry.

Conjugate

Unconjugated

Specificity

Does not react with mouse and rat.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

70 kDa lamin; Cardiomyopathy dilated 1A (autosomal dominant; CDCD1; CDDC; CMD1A; CMT2B1; EMD2; FPL; FPLD; FPLD2; HGPS; IDC; Lamin A; Lamin A/C; Lamin A/C like 1; Lamin; Lamin C; Lamin-A/C; LDP1; LFP; LGMD1B; Limb girdle muscular dystrophy 1B (autosomal dominant; LMN 1; LMN A; LMN C; LMN1; LMNA; LMNA_HUMAN; LMNC; LMNL1; Prelamin A/C; PRO1; Renal carcinoma antigen NY REN 32; Renal carcinoma antigen NY-REN-32; Renal carcinoma antigen NYREN32

UniProt ID

P02545
UniProt Summary: [Lamin-A/C]: Lamins are intermediate filament proteins that assemble into a filamentous meshwork, and which constitute the major components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane. Lamins provide a framework for the nuclear envelope, bridging the nuclear envelope and chromatin, thereby playing an important role in nuclear assembly, chromatin organization, nuclear membrane and telomere dynamics. Lamin A and C also regulate matrix stiffness by conferring nuclear mechanical properties. The structural integrity of the lamina is strictly controlled by the cell cycle, as seen by the disintegration and formation of the nuclear envelope in prophase and telophase, respectively. Lamin A and C are present in equal amounts in the lamina of mammals. Also involved in DNA repair: recruited by DNA repair proteins XRCC4 and IFFO1 to the DNA double-strand breaks (DSBs) to prevent chromosome translocation by immobilizing broken DNA ends. Required for normal development of peripheral nervous system and skeletal muscle and for muscle satellite cell proliferation. Required for osteoblastogenesis and bone formation. Also prevents fat infiltration of muscle and bone marrow, helping to maintain the volume and strength of skeletal muscle and bone. Required for cardiac homeostasis.

NCBI Gene ID

4000

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Anti-Lamin A/C Antibody (4C4)

SKU: M-1689-100

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