Anti-Lamin A/C Antibody (4C4)

Our Anti-Lamin A/C mouse monoclonal primary antibody detects human Lamin A/C, and is IgG. It is validated for use in FC, ICC, WB.

Left: HeLa cells stained with M-1689-100 (red), and counterstained with chicken polyclonal antibody to Vimentin (green, C-1409-50). The The Lamin A/C antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Right: Analysis of Lamin A/C expression in human prostate cancer DU145 cell line by FLow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Lamin A/C (cat # M-1689-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: Lamin A/C expression in HeLa cells, stained with mouse antibody to lamin A/C (red, 1:2,000) by Immunocytochemistry. Co-staining was performed with a rabbit anti-HSP60 antibody (green). Blue: Hoechst nuclear stain. The lamin A/C antibody specifically labels the nuclear lamina. Right: Western blot analysis of lamin A/C expression in cell lysates using mouse anti-lamin A/C antibody (green, 1:1,000). [1] protein standard, [2] HeLa, [3] HEK293 [4] C6, and [5] NIH-3T3 cell lysates. Two strong bands at 74 and 65 kDa correspond to the lamin A and lamin C proteins, respectively, detected only in the cells of human origin. The lamin A/C antibody does not recognize rat or mouse proteins.
Left: HeLa cells stained with M-1689-100 (red), and counterstained with chicken polyclonal antibody to Vimentin (green, C-1409-50). The The Lamin A/C antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Right: Analysis of Lamin A/C expression in human prostate cancer DU145 cell line by FLow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Lamin A/C (cat # M-1689-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Left: HeLa cells stained with M-1689-100 (red), and counterstained with chicken polyclonal antibody to Vimentin (green, C-1409-50). The The Lamin A/C antibody reveals strong nuclear lamina staining, while the Vimentin antibody reveals cytoplasmic intermediate filaments. The blue stain reveals DNA in the nuclei of these cells. Right: Analysis of Lamin A/C expression in human prostate cancer DU145 cell line by FLow Cytometry. Fixing and Permeabilization of cells: Absolute methanol (10 minutes in ice) and 0.1% Tween-20 in PBS, Blocking: 200 µg/mL Normal Sheep IgG (20 minutes), Primary antibody: Mouse Monoclonal antibody to Lamin A/C (cat # M-1689-100, 2 μg per ~10^6 cells) for 30 minutes at room temperature, Secondary antibody: Goat anti-mouse PE labeled secondary antibody (1:100 fold dilution) with incubation for 20 minutes in dark at room temperature. Non-specific Control IgG, clone X63 (cat # M-1249-100) was used as negative control under same conditions (black dashed). Flow cytometry data and results were generated using Orflo MoxiflowTM instrument and protocols.
Click on image to zoom

Human
Flow, ICC, WB
Mouse

Go to Full Product Details

Data Sheet

SKU: M-1689-100

Volume/Size: 100 µg
Price:
$327.00

Bulk Order Anti-Lamin A/C Antibody

Related Products

Recently Viewed