Immunofluorescence staining of adult wild-type (WT) and Kv2.2 knockout (KO) mouse magnocellular preoptic nucleus (MCPO) with N372B/1 (red) and Hoechst nuclear stain (blue).
Anti-Kv2.2 Potassium Channel Antibody
Bulk Order Anti-Kv2.2 Potassium Channel Antibody
Potassium voltage-gated channel subfamily B member 2 (CDRK) (Voltage-gated potassium channel subunit Kv2.2)
Voltage-gated K+ channels are important determinants of neuronal membrane excitability (Pongs, 1999). Moreover, differences in K+ channel expression patterns and densities contribute to the variations in action potential waveforms and repetitive firing patterns evident in different neuronal cell types. The delayed rectifier-type (IK)channels (Kv1.5, Kv2.1, and Kv2.2) are expressed on all neuronal somata and proximal dendrites and are also found in a wide variety of non-neuronal cells types including pancreatic islets, alveolar cells and cardiac myocytes (Hwang et al., 1993; Yan et al., 2004; Michaelevski et al., 2003). Kv2.1 and Kv2.2 form distinct populations of K+ channels and these subunits are thought to be primarily responsible for IK in superior cervical ganglion cells (Blaine and Ribera, 1998; Burger and Ribera, 1996).
ICC, IHC, WB
Fusion protein amino acids 717-907 (cytoplasmic C-terminus) of rat Kv2.2 long isoform (also known as Potassium voltage-gated channel subfamily B member 2, Kcnb2 and CDRK, accession number Q63099); Mouse: 94% identity (180/191 amino acids identical); Human: 84% identity (161/191 amino acids identical); 50% identity with Kv2.1 and other Kv2 channels100% identity with Kv2.2 short isoform for first 47 amino acids (ENRGSAPQTPPSTARPLPVTTADFPLTTPQHMSTILLEEALPQGQRP)
Human, Mouse, Rat
Store at ≤ -20 C for long term storage. For short term storage, store at 2-8 C. For maximum recovery of product, centrifuge the vial prior to removing the cap.