Immunoblot versus lysates of total brain from HCN3 knockout (KO) and wild-type (WT) mice probed with N141/28. Data courtesy of Martin Biel (Ludwig-Maximilians Universität München) and reproduced from the Journal of Biological Chemistry (2013 Cao-Ehlker et al, PMID 23382386).
HCN3 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations HCN3 is found in brain, heart, skeletal muscle and lung.
ICC, IHC, WB
Fusion protein amino acids 660-779 (cytoplasmic C-terminus) of mouse HCN3(also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3, hyperpolarization-activated cation channel 3 and HAC-3, accession number O88705); Rat: 98% identity (118/120 amino acids identical)
Human: 86% identity (105/121 amino acids identical)
Less than 50% identity with HCN1, HCN2 and HCN4
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