Adult rat and mouse brain membrane (RBM and MBM, respectively) and transfected cell immunoblot: extracts of RBM, HCN1-KO and -WT MBM and COS-1 cells transfected with GFP-HCN1, GFP-HCN2 or Kv2.1 plasmids and probed with N70/28 (left panel) or K89/34 TC supe (right panel).
HCN1 is a member of the hyperpolarization-activated cyclic nucleotide gated channel family, also known as pacemaker channels. This family has a role in rhythmic activity of the heart and firing neurons. HCN channels are made up of four subunits, HCN1-4 and can be formed in multiple combinations. HCN1 is found in the hippocampus, neocortex, and cerebellar cortex of brain. Alterations in this protein have been implicated in the disease early infantile epileptic encephalopathy.
IHC, IP, WB
Fusion protein amino acids 778-910 (cytoplasmic C-terminus) of rat HCN1 (also known as potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1, accession number Q9JKB0); Mouse: 96% identity (128/133 amino acids identical); Human: 90% identity (121/133 amino acids identical); Less than 50% identity with HCN2, HCN3 and HCN4
Human, Mouse, Rat
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