Anti-Growth associated protein 43 (GAP-43) Antibody

Name: Anti-Growth associated protein 43 (GAP-43) Antibody
Brand: Biosensis
SKU: C-1649-100
Price: 327.0 USD
Availability: InStock

Our Anti-Growth associated protein 43 (GAP-43) chicken polyclonal primary antibody detects bovine, human, other mammals (predicted), and rat Growth associated protein 43 (GAP-43), and is concentrated purified IgY. It is validated for use in ICC, WB.

A: Immunofluorescence analysis of GAP43 expression (green) in mixed neuron-glial cultures with C-1649-100. GAP43-IR is observed in axons and dendrites, with strong staining of the submembraneous cytoskeleton and vesicles. Cultures were co-labelled with R-1694-100, a rabbit polyclonal antibody to alpha-II-spectrin (red), and a DNA dye (blue).B: Western blot of cow hippocampus extract. C-1649-100 detects GAP43 at its expected molecular weight of ~43 kDa.

Left: Analysis of GAP43 expression in cortical neuron-glial cell culture from E20 rat by Immunocytochemistry. Staining was performed with chicken antibody to GAP43 (1:2,000, green) and rabbit antibody to alpha-II spectrin (R-1694-100, 1:1,000, red). Blue: DAPI nuclear stain. The GAP43 antibody labels protein expressed in the axonal membrane and synapses of neuronal cells, while the spectrin antibody stains the submembraneous cytoskeleton of axons and dendrites. Right: Western blot analysis of tissue and cell lysates using chicken antibody to GAP43 (1:5,000). [1] protein standard, [2] rat brain, [3] rat spinal cord, [4] mouse brain, [5] mouse spinal cord, [6] C6 cells, [7] SH-SY5Y cells. A single band at 43 kDa corresponds to GAP43. The GAP43 protein is only detected in lysates of neuronal origin. C6 rat glioma cells [6] do not express GAP43.

A: Immunofluorescence analysis of GAP43 expression (green) in mixed neuron-glial cultures with C-1649-100. GAP43-IR is observed in axons and dendrites, with strong staining of the submembraneous cytoskeleton and vesicles. Cultures were co-labelled with R-1694-100, a rabbit polyclonal antibody to alpha-II-spectrin (red), and a DNA dye (blue).
B: Western blot of cow hippocampus extract. C-1649-100 detects GAP43 at its expected molecular weight of ~43 kDa.

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Species Reactivity

Bovine, Human, Rat

Applications

ICC, WB

Host Species

Chicken

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Data Sheet

SKU: C-1649-100

Volume/Size: 100 µL

Variant

Price:

$327.00

Quantity:

Product Details

Target

Growth associated protein 43 (GAP-43)

Scientific Background

GAP43 is very abundant protein which is found concentrated in neurons. One group discovered it as one of three proteins which becomes unregulated during the regeneration of the toad optic nerve (1). Three GAPs (Growth associated proteins) were discovered, and the number 43 comes from the apparent SDS-PAGE molecular weight of the one named GAP43. The HGNC name for this protein is, not surprisingly, GAP43. Later work showed that GAP43 does not run on SDS-PAGE in a fashion which accurately reflects its molecular weight, and that GAP43 proteins from different species may run at different apparent molecular weights. Partly due to these features GAP43 were independently discovered by several different groups and therefore has several alternate names, such as protein F1, pp46, neuromodulin, neural phosphoprotein B-50 and calmodulin-binding protein P-57. In each case the number reflects the apparent SDS-PAGE molecular weight, and underlines the unusual properties of this molecule. Mammalian GAP43 proteins contains only 226-243 amino acids, and so the real molecular weight is 23.61-25.14 kDa. GAP43 has been extensively studied and is known to be a major protein kinase C substrate and to bind calmodulin avidly. GAP43 is anchored to the plasma membrane by palmitoylation modifications.

Format

Concentrated purified IgY

Clonality

Polyclonal

Isotype

IgY

Applications

ICC, WB

Host Species

Chicken

Molecular Weight

43 kDa

Immunogen

C-terminal peptide 217-227 of rat and mouse GAP43, which is KEDPEADQEHA, to which an N terminal Cysteine residue was added to allow chemical coupling to Keyhole Limpet Hemocyanin carrier protein.

Immunogen Species

Rat

Species Reactivity

Bovine, Human, Rat

Storage

Spin vial briefly before opening. Reconstitute with 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. Store lyophilized, unopened vial at 2-8°C or lower. After reconstitution, prepare aliquots and store at -20°C to -80°C for a higher stability. Avoid freeze-thaw cycles.

Physical State

Lyophilized

Buffer

Lyophilized IgY preparation, with sodium azide.

Dilution Ranges

WB: 1:1000-1:5000
ICC: 1:500-1:1000

Conjugate

Unconjugated

Specificity

The antibody reacts with a 43 kDa band by Western blot on bovine hippocampus lysate. It has also been used successfully for immunocytochemistry. Human, rat and bovine. It is expected that it will work on other mammal tissues due to amino acid sequence similarity.

Usage Statement

For research use only.

Country of Origin

United States

Expiration

12 months after date of receipt (unopened vial).

Synonyms

neuron growth-associated protein 43; neuromodulin; nerve growth-related peptide GAP43; axonal membrane protein GAP-43; protein F1; calmodulin-binding protein P-57; neural phosphoprotein B-50; Growth Associated Protein 43; GAP43;

UniProt ID

P07936

Shipping

25°C (ambient)

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Anti-Growth associated protein 43 (GAP-43) Antibody

SKU: C-1649-100

Product Details

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