Our Anti-GAD67 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone L127/8. It detects human, mouse, and rat GAD67, and is purified by Protein A chromatography. It is great for use in AT, IHC, ICC, WB.
Western blotting of rat brain homogenate (10 ug/lane) with anti-GAD65/67 (L127/8) at 1:100 dilution and detected with anti-mouse HRP.
Glutamate decarboxylase 1, GAD67 or GAD1 is a member of the group II decarboxylase family and catalyzes the production of GABA from L-glutamic acid. GAD67 is expressed in brain (throughout the cell), in insulin producing ßcells of the pancreas, and several non-neuronal tissues during development. Alterations in GAD67 have been shown to lead to pyridoxine dependency with seizures. Other disorders associated with GAD67 are Cerebral Palsy, Spastic Quadriplegic, 1 and Inherited Congenital Spastic Tetraplegia.
Purified by Protein A chromatography
1 mg/mL
Monoclonal
L127/8
IgG2b
ICC, IHC, WB
Mouse
GAD1, GAD, GAD67
70 kDa
Fusion protein amino acids 1-594 (full-length) of human GAD67 (accession number Q99259) produced recombinantly in E. Coli
Human, Mouse, Rat
AB_2756509
Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.
Liquid
Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.
10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.125
Unconjugated
Cross-reacts with GAD25 Does not cross-react with GAD44 or GAD65/GAD2
Each new lot of this antibody is tested to confirm that it recognizes a single immunoreactive band of expected molecular weight when used to probe brain lysate.
These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.
