Our Anti-GABAA Receptor α6 primary antibody from PhosphoSolutions is rabbit polyclonal. It detects mouse and rat GABAA Receptor α6 and is neat pooled serum. It is great for use in WB.
Western blot of rat cortical lysate showing specific immunolabeling of the ~57 kDa α6-subunit of the GABAA-R.
Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system, causing a hyperpolarization of the membrane through the opening of a Cl− channel associated with the GABA-A receptor (GABA-A-R) subtype. GABA-A-Rs are important therapeutic targets for a range of sedative, anxiolytic, and hypnotic agents and are implicated in several diseases including epilepsy, anxiety, depression, and substance abuse. The GABA-A-R is a multimeric subunit complex. To date six αs, four βs and four γs, plus alternative splicing variants of some of these subunits, have been identified (Olsen and Tobin, 1990; Whiting et al., 1999; Ogris et al., 2004). Injection in oocytes or mammalian cell lines of cRNA coding for α- and β-subunits results in the expression of functional GABA-A-Rs sensitive to GABA. However, coexpression of a γ-subunit is required for benzodiazepine modulation. The various effects of the benzodiazepines in brain may also be mediated via different α-subunits of the receptor (McKernan et al., 2000; Mehta and Ticku, 1998; Ogris et al., 2004; Pöltl et al., 2003). Lastly, phosphorylation of β-subunits of the receptor has been shown to modulate GABAA-R function (Brandon et al., 2003).
Neat Pooled Serum
Polyclonal
IgG
WB
Rabbit
GABRA6
57 kDa
Synthetic peptide corresponding to amino acid residues specific to the α6 subunit of rat GABAA receptor, conjugated to keyhole limpet hemocyanin (KLH).
Mouse, Rat
AB_2492106
Recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) upon arrival and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.
Liquid
Neat serum
Neat Serum
WB: 1:1000
WB Brain: 1:1000
Unconjugated
Specific for endogenous levels of the ~57 kDa α6-subunit of the GABAA receptor.
Western blots performed on each lot.
For research use only. Not intended for therapeutic or diagnostic use. Use of all products is subject to our terms and conditions, which can be viewed on our website.
After date of receipt, stable for at least 1 year at -20°C.
Blue Ice
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Brandon NJ, et al. (2003) A kinase anchoring protein 79/150 facilitates the phosphorylation of GABAA receptors by cAMP-dependent protein kinase via selective interaction with receptor β-subunits. Mol Cell Neurosci 22:87-97. PMID: 12595241
McKernan RM, et al. (2000) Sedative but not anxiolytic properties of benzodiazepines are mediated by the GABAA receptor α1-subtype. Nature Neurosci 3:587-592. PMID: 10816315
Mehta AK, Ticku MK (1998) Prevalence of the GABAA receptor assemblies containing α1-subunit in the rat cerebellum and cerebral cortex as determined by immunoprecipitation: Lack of modulation by chronic ethanol administration. Mol Brain Res 67:194-199. PMID: 10101248
Ogris W, et al.(2004) Affinity of various benzodiazepine site ligands in mice with a point mutation in the GABAA receptor γ2-subunit. Biochem Pharmacol 68:1621-1629. Olsen RW, Tobin AJ (1990) Molecular biology of GABAA receptors. FASEB 4:1469-1480. PMID: 15451405
Pöltl A, et al. (2003) Subunit composition and quantitative importance of GABAA receptor subtypes in the cerebellum of mouse and rat. J Neurochem 87:1444-1455. PMID: 14713300
Whiting PJ, et al. (1999) Molecular and functional diversity of the expanding GABAA receptor gene family. Ann NY Acad Sci 868:645-653. PMID: 10414349
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