Anti-GABA-B-R2 Antibody (N81/37)

Name: Anti-GABA-B-R2 Antibody
Brand: NeuroMab
SKU: 75-125
Price: 329.0 USD
Availability: InStock

Our Anti-GABA(B)R2 mouse monoclonal primary antibody from NeuroMab is produced in-house from hybridoma clone N81/37. It is KO validated, detects human and mouse GABA(B)R2, and is purified by Protein A chromatography. It is great for use in IHC, ICC, WB.

$Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.$

$Immunoblot against brain membrane fractions from rat (RBM) and from GABABR2 knockout (KO) and wild-type (WT) mice probed with N81/37 (left) or N52A/42 (right) TC supe. Mouse brains courtesy of Joshua Walker and Stephen Moss (Tufts).$

Electron micrograph of N81/37 labelling in stratum radiatum of mouse hippocampal CA1 using a pre-embedding immunogold method. Immunoparticles are seen at postsynaptic sites (arrows) along the extrasynaptic plasma membrane of dendritic spines (s) and shafts of pyramidal cells forming synaptic contacts with excitatory axon terminals (b), and at presynaptic sites (arrowheads) in excitatory axon terminals (b). Image courtesy of Rafael Lujan (Universidad de Castilla-La Mancha).

Immunoblot against membrane fractions from whole mouse (MBM) or rat (RBM) brain and from human hippocampus [HBM(H)], cerebral cortex [HBM(Cx)] or cerebellum [HBM(Cb)] and probed with N81/37 (left) or N52A/42 (right) TC supe.

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Species Reactivity

Human, Mouse

Applications

ICC, IHC, WB

Host Species

Mouse

Conjugates and Formulations

490

550

594

650

Carrier-Free

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Data Sheet

KO Validated

SKU: 75-125

Volume: 100 µL

Variant

Price:

$329.00

Quantity:

Ships: 1-2 business days

Product Details

Target

GABA(B)R2

Scientific Background

Gamma-aminobutyric acid (GABA) is the primary inhibitory neurotransmitter in the central nervous system. There are two major classes of GABA receptors: the GABAA and the GABAB subtype of receptors. GABAB receptors are heterodimeric G protein-coupled receptors that mediate slow synaptic inhibition in the central nervous system. It has recently been demonstrated that AMPK binds directly to GABAB receptors.

Format

Purified by Protein A chromatography

Concentration

1 mg/mL

Clonality

Monoclonal

Clone

N81/37

Isotype

IgG1

Applications

ICC, IHC, WB

Host Species

Mouse

Gene Name

GABBR2 GPR51 GPRC3B

Molecular Weight

105 kDa

Antigen

Fusion protein amino acids 861-912 of rat GABABR2 (accession number NP_113990) produced recombinantly in E. Coli

Species Reactivity

Human, Mouse

Antibody Registry ID

AB_2278802

Storage

Aliquot and store at ≤ -20°C for long term storage. For short term storage, store at 2-8°C. For maximum recovery of product, centrifuge the vial prior to removing the cap.

Physical State

Liquid

Production Notes

Produced by in vitro bioreactor culture of hybridoma line followed by Protein A affinity chromatography. Purified mAbs are >90% specific antibody.

Buffer

10 mM Tris, 50 mM Sodium Chloride, 0.065% Sodium Azide pH 7.52

Conjugate

Unconjugated

Specificity

No cross-reactivity against GABABR1

Quality Control

Each new lot of antibody is quality control tested by western blot on rat whole brain lysate and confirmed to stain the expected molecular weight band.

Usage Statement

These antibodies are to be used as research laboratory reagents and are not for use as diagnostic or therapeutic reagents in humans.

Country of Origin

United States

Expiration

24 months from date of receipt

Shipping

Shipped on ice packs

Synonyms

Gamma-aminobutyric acid type B receptor subunit 2 (GABA-B receptor 2) (GABA-B-R2) (GABA-BR2) (GABABR2) (Gb2) (G-protein coupled receptor 51) (HG20)

UniProt ID

O75899

Product Specific References for Applications and Species

Co- Immunoprecipitation: Mouse
Immunohistochemistry: Mouse
Immunoprecipitation: Mouse
Western Blot: Mouse
Additional Publications: Unspecified

Co-Immunoprecipitation: Mouse
PMID	Dilution	Publication
19558451	not listed	Fernández-Alacid, L., et al. 2009. Subcellular compartment-specific molecular diversity of pre- and post-synaptic GABA-activated GIRK channels in Purkinje cells.. Journal of Neurochemistry, 1363-1376.

Immunohistochemistry: Mouse
PMID	Dilution	Publication
33888718	1:500-1:1000	Luo, F., et al. 2021. Neurexins regulate presynaptic GABAB-receptors at central synapses. Nature Communications, 2380.

Immunoprecipitation: Mouse
PMID	Dilution	Publication
24431439	not listed	Terunuma, M., et al. 2014. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory.. Journal of Neuroscience, 804-816.

Western Blot: Mouse
PMID	Dilution	Publication
30315127	not listed	Ostrovskaya, O.I., et al. 2018. Inhibitory signaling to ion channels in hippocampal neurons is differentially regulated by alternative macromolecular complexes of RGS7.. Journal of Neuroscience, 10002-10015.
25843643	1:10 (supe)	Marron Fernandez de Velasco, E., et al. 2014. Sex differences in GABA(B)R-GIRK signaling in layer 5/6 pyramidal neurons of the mouse prelimbic cortex.. Neuropharmacology, 353-360.
25172509	not listed	Huyghe, D., et al. 2014. Glutamine synthetase stability and subcellular distribution in astrocytes are regulated by γ-aminobutyric type B receptors. . Journal of Biological Chemistry, 28808-28815.
24431439	not listed	Terunuma, M., et al. 2014. Postsynaptic GABAB receptor activity regulates excitatory neuronal architecture and spatial memory.. Journal of Neuroscience, 804-816.
24094109	1:10 (supe)	Hearing, M., et al. 2013. Repeated cocaine weakens GABA(B)-Girk signaling in layer 5/6 pyramidal neurons in the prelimbic cortex.. Neuron, 159-170.
22405207	not listed	Padgett, C.L., et al. 2013. Methamphetamine-evoked depression of GABA(B) receptor signaling in GABA neurons of the VTA.. Neuron, 978-989.

Additional Publications: Unspecified
PMID	Publication
21336828	Broussard, D.M., et al. 2011. Motor learning in the VOR: the cerebellar component.. Experimental Brain Research, 451-463.

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Anti-GABA-B-R2 Antibody (N81/37)

SKU: 75-125

Product Details

Product Specific References for Applications and Species

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